验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, HT-1080 cells, SK-N-SH cells, NIH/3T3 cells |
| Positive IHC detected in | human ovarian cancer Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive FC (Intra) detected in | HepG2 cells |
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
83689-3-RR targets HSP47/SERPINH1 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.
| 经测试应用 | WB, IHC, FC (Intra), ELISA Application Description |
| 经测试反应性 | human, mouse |
| 免疫原 | HSP47/SERPINH1 fusion protein Eg1201 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1) |
| 别名 | HSP47, SERPINH1, 240752F6, 47 kDa heat shock protein, Arsenic-transactivated protein 3 |
| 计算分子量 | 46kDa |
| 观测分子量 | 46 kDa |
| GenBank蛋白编号 | NM_001235.5 |
| 基因名称 | HSP47/SERPINH1 |
| Gene ID (NCBI) | 871 |
| RRID | AB_3671291 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein A purfication |
| UNIPROT ID | P50454 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSP47/SERPINH1 antibody 83689-3-RR | Download protocol |
| IHC protocol for HSP47/SERPINH1 antibody 83689-3-RR | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |