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验证数据展示

  • FC experiment of HepG2 using CL647-10875

    FC experiment of HepG2 using CL647-10875

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 647 Anti-Human HSP47 (CL647-10875) (red), or 0.4 ug CoraLite® Plus 647-conjugated Rabbit IgG control Rabbit PolyAb (CL647-30000, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated HSP47/SERPINH1 Polyclonal antibody

HSP47/SERPINH1 Polyclonal Antibody for FC (Intra)
Cat No. CL647-10875

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

FC (Intra)

别名

HSP47, SERPINH1, Cell proliferation-inducing gene 14 protein, CBP2, CBP1

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10875-1-AP

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10875 targets HSP47/SERPINH1 in FC (Intra) applications and shows reactivity with human, mouse samples.

经测试应用 FC (Intra) Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag1301

Product name: Recombinant human SERPINH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-418 aa of BC014623

Sequence: MRSLLLLSAFCLLEAALAAEVKKPAAAAAPGTAEKLSPKAATLAERSAGLAFSLYQAMAKDQAVENILVSPVVVASSLGLVSLGGKATTASQAKAVLSAEQLRDEEVHAGLGELLRSLSNSTARNVTWKLGSRLYGPSSVSFADDFVRSSKQHYNCEHSKINFRDKRSALQSINEWAAQTTDGKLPEVTKDVERTDGALLVNAMFFKPHWDEKFHHKMVDNRGFMVTRSYTVGVMMMHRTGLYNYYDDEKEKLQIVEMPLAHKLSSLIILMPHHVEPLERLEKLLTKEQLKIWMGKMQKKAVAISLPKGVVEVTHDLQKHLAGLGLTEAIDKNKADLSRMSGKKDLYLASVFHATAFELDTDGNPFDQDIYGREELRSPKLFYADHPFIFLVRDTQSGSLLFIGRLVRPKGDKMRDEL

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
别名 HSP47, SERPINH1, Cell proliferation-inducing gene 14 protein, CBP2, CBP1
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC014623
基因名称 HSP47/SERPINH1
Gene ID (NCBI) 871
RRIDAB_2934837
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50454
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 HSP47/SERPINH1 antibody CL647-10875Download protocol
Standard Protocols
Click here to view our Standard Protocols