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验证数据展示

  • WB analysis using 10875-1-AP

    WB analysis using 10875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis using 10875-1-AP

    WB analysis using 10875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 10875-1-AP

    WB analysis of HeLa using 10875-1-AP

    WB result of HSP47 antibody (10875-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HSP47 transfected HeLa cells.

  • WB analysis using 10875-1-AP

    WB analysis using 10875-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10875-1-AP (HSP47/SERPINH1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human stomach cancer using 10875-1-AP

    IHC staining of human stomach cancer using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human stomach cancer using 10875-1-AP

    IHC staining of human stomach cancer using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of mouse kidney using 10875-1-AP

    IHC staining of mouse kidney using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of mouse lung using 10875-1-AP

    IHC staining of mouse lung using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of rat kidney using 10875-1-AP

    IHC staining of rat kidney using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of rat lung using 10875-1-AP

    IHC staining of rat lung using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded rat lung tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human colon using 10875-1-AP

    IHC staining of human colon using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human colon using 10875-1-AP

    IHC staining of human colon using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human lung cancer using 10875-1-AP

    IHC staining of human lung cancer using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IHC staining of human lung cancer using 10875-1-AP

    IHC staining of human lung cancer using 10875-1-AP

    Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10875-1-AP (HSP47 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HSP47 antibody included
  • IF Staining of HeLa using 10875-1-AP

    IF Staining of HeLa using 10875-1-AP

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HSP47 antibody (10875-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • FC experiment of HepG2 using 10875-1-AP

    FC experiment of HepG2 using 10875-1-AP

    1X10^6 HepG2 cells were intracellularly stained with 0.4 ug Anti-Human HSP47 (10875-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Featured Product
  • KD/KO验证

HSP47/SERPINH1 Polyclonal antibody

HSP47/SERPINH1 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 10875-1-AP

产品说明书

引用文献(14)

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), ELISA and More (2)

别名

HSP47, SERPINH1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inHT-1080 cells, A2780 cells, A431 cells, HeLa cells, NIH/3T3 cells, BALB/3T3 clone A31 cells
Positive IHC detected inhuman stomach cancer tissue, human colon tissue, human hepatocirrhosis tissue, human lung cancer tissue, mouse kidney tissue, mouse lung tissue, rat kidney tissue, rat lung tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HSP47 Ready-To-Use IHC Kit. HSP47 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite® Plus 647 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

10875-1-AP targets HSP47/SERPINH1 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC, IF, IP
经测试反应性 human, mouse, rat
文献引用反应性human, mouse
免疫原 HSP47/SERPINH1 fusion protein Ag1301 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 serpin peptidase inhibitor, clade H (heat shock protein 47), member 1, (collagen binding protein 1)
别名 HSP47, SERPINH1, 47 kDa heat shock protein, Arsenic-transactivated protein 3, AsTP3
计算分子量 46 kDa
观测分子量 46 kDa
GenBank蛋白编号BC014623
基因名称 HSP47/SERPINH1
Gene ID (NCBI) 871
RRIDAB_2301500
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP50454
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSP47 is also named as Serpin H1, colligen, CBP1, CBP2, HSP47, SERPINH2 and belongs to the serpin family. This gene encodes a member of the serpin superfamily of serine proteinase inhibitors. The encoded protein is localized to the endoplasmic reticulum. It binds specifically to collagen and can be involved as a chaperone in the biosynthetic pathway of collagen. The Serpin Family H Member 1 (SERPINH1) gene encodes a protein called HSP47, which is required for the correct folding and secretion of collagen (PMID: 30128672). SERPINH1 has been implicated in several collagen-related diseases, such as, brittle bone disease, keloid scars, and lung fibrosis (PMID: 32091407). SERPINH1 also correlates with the grade of glioma, and regulates proliferation, migration and invasion of glioma cell lines (PMID: 24595468). SERPINH1 regulates the Wnt/β-catenin signaling pathway and modulates gastric cancer (GC) progression (PMID: 32091407). SERPINH1 is aberrantly expressed in cervical cancer, breast cancer, glioblastoma and colorectal cancer (PMID: 32091407). SERPINH1 promotes invasion and metastasis of breast cancer cells by regulating the expression of several extracellular matrix (ECM) proteins (PMID: 25744716). Autoantibodies to the encoded protein have been found in patients with rheumatoid arthritis. HSP47 has a calculated molecular weight of 46 kDa.

实验方案

Product Specific Protocols
WB protocol for HSP47/SERPINH1 antibody 10875-1-APDownload protocol
IHC protocol for HSP47/SERPINH1 antibody 10875-1-APDownload protocol
IF protocol for HSP47/SERPINH1 antibody 10875-1-APDownload protocol
FC protocol for HSP47/SERPINH1 antibody 10875-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Nat Protoc

Responsive and activable nanomedicines for remodeling the tumor microenvironment.

Authors - Yinlong Zhang
  • KD Validated
View Article
humanWB,IF,IHC

Nat Commun

Reversal of pancreatic desmoplasia by re-educating stellate cells with a tumour microenvironment-activated nanosystem.

Authors - Xuexiang Han
  • KD Validated
View Article
human,mouseWB,IHC

Cancer Res

RUNX1 is a driver of renal cell carcinoma correlating with clinical outcome.

Authors - Nicholas Rooney
View Article
humanIP

mSystems

Proteomic Profiling Identifies Kaposi's Sarcoma-Associated Herpesvirus (KSHV)-Encoded LANASIM-Associated Proteins in Hypoxia.

Authors - Xiaoqing Liu
View Article
mouseWB

PLoS Genet

Cyclophilin B control of lysine post-translational modifications of skin type I collagen.

Authors - Masahiko Terajima
View Article
mouseWB

Sci Rep

Lysyl hydroxylase 2 mediated collagen post-translational modifications and functional outcomes

Authors - Masahiko Terajima
View Article