验证数据展示
经过测试的应用
| Positive WB detected in | rat liver tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| WB | See 3 publications below |
产品信息
15132-1-AP targets HSCB in WB, IHC, ELISA applications and shows reactivity with human, rat samples.
| 经测试应用 | WB, IHC, ELISA Application Description |
| 文献引用应用 | WB |
| 经测试反应性 | human, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag7268 Product name: Recombinant human HSCB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-235 aa of BC065569 Sequence: MWRGRAGALLRVWGFWPTGVPRRRPLSCDAASQAGSNYPRCWNCGGPWGPGREDRFFCPQCRALQAPDPTRDYFSLMDCNRSFRVDTAKLQHRYQQLQRLVHPDFFSQRSQTEKDFSEKHSTLVNDAYKTLLAPLSRGLYLLKLHGIEIPERTDYEMDRQFLIEIMEINEKLAEAESEAAMKEIESIVKAKQKEFTDNVSSAFEQDDFEEAKEILTKMRYFSNIEEKIKLKKIPL 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | HscB iron-sulfur cluster co-chaperone homolog (E. coli) |
| 别名 | JAC1, Iron-sulfur cluster co-chaperone protein HscB, mitochondrial, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic, Iron-sulfur cluster co-chaperone protein HscB, HSC20 |
| 计算分子量 | 27 kDa |
| 观测分子量 | 27-30 kDa |
| GenBank蛋白编号 | BC065569 |
| 基因名称 | HSCB |
| Gene ID (NCBI) | 150274 |
| RRID | AB_2878110 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | Q8IWL3 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HSCB, also named as HSC20 and DNAJC20, acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria.
实验方案
| Product Specific Protocols | |
|---|---|
| WB protocol for HSCB antibody 15132-1-AP | Download protocol |
| IHC protocol for HSCB antibody 15132-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Cancers (Basel) Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin | ||
Mol Cell Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis |