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验证数据展示

  • WB analysis of rat liver using 15132-1-AP

    WB analysis of rat liver using 15132-1-AP

    rat liver tissue were subjected to SDS PAGE followed by western blot with 15132-1-AP (HSCB antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • IHC staining of human colon cancer using 15132-1-AP

    IHC staining of human colon cancer using 15132-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15132-1-AP (HSCB antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

HSCB Polyclonal antibody

HSCB Polyclonal Antibody for WB, IHC, ELISA
Cat No. 15132-1-AP

产品说明书

引用文献(3)

宿主/亚型

Rabbit / IgG

种属反应性

human, rat

应用

WB, IHC, ELISA

别名

JAC1, Iron-sulfur cluster co-chaperone protein HscB, mitochondrial, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic, Iron-sulfur cluster co-chaperone protein HscB, HSC20

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
规格: 

-/ -

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经过测试的应用

Positive WB detected inrat liver tissue
Positive IHC detected inhuman colon cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 3 publications below

产品信息

15132-1-AP targets HSCB in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

经测试应用 WB, IHC, ELISA Application Description
文献引用应用WB
经测试反应性 human, rat
文献引用反应性human
免疫原 HSCB fusion protein Ag7268 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HscB iron-sulfur cluster co-chaperone homolog (E. coli)
别名 JAC1, Iron-sulfur cluster co-chaperone protein HscB, mitochondrial, Iron-sulfur cluster co-chaperone protein HscB, cytoplasmic, Iron-sulfur cluster co-chaperone protein HscB, HSC20
计算分子量 27 kDa
观测分子量 27-30 kDa
GenBank蛋白编号BC065569
基因名称 HSCB
Gene ID (NCBI) 150274
RRIDAB_2878110
偶联类型 Unconjugated
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IWL3
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HSCB, also named as HSC20 and DNAJC20, acts as a co-chaperone in iron-sulfur cluster assembly in mitochondria.

实验方案

Product Specific Protocols
WB protocol for HSCB antibody 15132-1-APDownload protocol
IHC protocol for HSCB antibody 15132-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

Cancers (Basel)

Mutant p53 Depletion by Novel Inhibitors for HSP40/J-Domain Proteins Derived from the Natural Compound Plumbagin

Authors - Mohamed Alalem
View Article
humanWB

Science

Autoregulatory control of mitochondrial glutathione homeostasis

Authors - Yuyang Liu
View Article
humanWB

Mol Cell

Dual regulation of SLC25A39 by AFG3L2 and iron controls mitochondrial glutathione homeostasis

Authors - Xiaojian Shi
View Article