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CoraLite®594-conjugated HPGD Monoclonal antibody

HPGD Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3F1F5

Cat No : CL594-66798

Print datasheet

Synonyms

15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1

验证数据展示

  • IF Staining of A549 using CL594-66798

    IF Staining of A549 using CL594-66798

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using CoraLite®594 HPGD antibody (CL594-66798, Clone: 3F1F5 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inA549 cells
For other applications, we recommend the unconjugated version of this antibody, 66798-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66798 targets HPGD in IF applications and shows reactivity with Human samples.

Tested Applications IF/ICC
Tested Reactivity Human
Immunogen HPGD fusion protein Ag1499 种属同源性预测
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Full Name hydroxyprostaglandin dehydrogenase 15-(NAD)
Synonyms 15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1
Calculated Molecular Weight 29 kDa
Observed Molecular Weight 25-28 kDa
GenBank Accession NumberBC018986
Gene Symbol HPGD
Gene ID (NCBI) 3248
RRIDAB_2920040
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP15428
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.

实验方案

Product Specific Protocols
IF protocol for CL594 HPGD antibody CL594-66798Download protocol
Standard Protocols
Click here to view our Standard Protocols