HPGD Monoclonal antibody

HPGD Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 66798-1-Ig

产品说明书

CloneNo. 3F1F5

宿主/亚型

Mouse / IgG2b

种属反应性

Human and More (1)

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite®594
规格: 

-/ -


经过测试的应用

Positive WB detected inCOLO 320 cells, human small intestine tissue, Caco-2 cells, HT-29 cells, human colon tissue
Positive IHC detected inhuman colon tissue, human colon cancer tissue, human lung cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inA549 cells
Positive FC (Intra) detected inA549 cells
Positive FC detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy HPGD Ready-To-Use IHC Kit. HPGD primary antibody included.
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66798-1-Ig targets HPGD in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
文献引用应用WB, IHC
经测试反应性 Human
文献引用反应性human
免疫原 HPGD fusion protein Ag1499 种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 hydroxyprostaglandin dehydrogenase 15-(NAD)
别名 15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1
计算分子量 29 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC018986
基因名称 HPGD
Gene ID (NCBI) 3248
RRIDAB_2861358
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP15428
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.

实验方案

Product Specific Protocols
WB protocol for HPGD antibody 66798-1-IgDownload protocol
IHC protocol for HPGD antibody 66798-1-IgDownload protocol
IF protocol for HPGD antibody 66798-1-IgDownload protocol
FC protocol for HPGD antibody 66798-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanWB

PLoS One

Molecular mechanism of inhibitory effects of melatonin on prostate cancer cell proliferation, migration and invasion.

Authors - Altannavch Nyamsambuu
humanWB,IHC

Nat Chem Biol

ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism

Authors - Linchong Sun
  • KD Validated
humanWB,IHC

Reprod Sci

Progressively Diminished Prostaglandin E2 Signaling in Concordance with Increasing Fibrosis in Ectopic Endometrium

Authors - Yunhua Yi
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