验证数据展示
经过测试的应用
Positive WB detected in | COLO 320 cells, human small intestine tissue, Caco-2 cells, HT-29 cells, human colon tissue |
Positive IHC detected in | human colon tissue, human colon cancer tissue, human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | A549 cells |
Positive FC (Intra) detected in | A549 cells |
Positive FC detected in | A549 cells |
推荐稀释比
应用 | 推荐稀释比 |
---|---|
Western Blot (WB) | WB : 1:5000-1:50000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension |
Flow Cytometry (FC) | FC : 0.20 ug per 10^6 cells in a 100 µl suspension |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
发表文章中的应用
KD/KO | See 1 publications below |
WB | See 3 publications below |
IHC | See 2 publications below |
产品信息
66798-1-Ig targets HPGD in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples.
经测试应用 | WB, IHC, IF/ICC, FC (Intra), ELISA Application Description |
文献引用应用 | WB, IHC |
经测试反应性 | Human |
文献引用反应性 | human |
免疫原 | HPGD fusion protein Ag1499 种属同源性预测 |
宿主/亚型 | Mouse / IgG2b |
抗体类别 | Monoclonal |
产品类型 | Antibody |
全称 | hydroxyprostaglandin dehydrogenase 15-(NAD) |
别名 | 15 PGDH, HPGD, PGDH, PGDH1, Prostaglandin dehydrogenase 1, SDR36C1 |
计算分子量 | 29 kDa |
观测分子量 | 25-28 kDa |
GenBank蛋白编号 | BC018986 |
基因名称 | HPGD |
Gene ID (NCBI) | 3248 |
RRID | AB_2861358 |
偶联类型 | Unconjugated |
形式 | Liquid |
纯化方式 | Protein A purification |
UNIPROT ID | P15428 |
储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol , pH 7.3 |
储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.
实验方案
Product Specific Protocols | |
---|---|
WB protocol for HPGD antibody 66798-1-Ig | Download protocol |
IHC protocol for HPGD antibody 66798-1-Ig | Download protocol |
IF protocol for HPGD antibody 66798-1-Ig | Download protocol |
FC protocol for HPGD antibody 66798-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
PLoS One Molecular mechanism of inhibitory effects of melatonin on prostate cancer cell proliferation, migration and invasion. | ||
Nat Chem Biol ENO1 promotes liver carcinogenesis through YAP1-dependent arachidonic acid metabolism
| ||
Reprod Sci Progressively Diminished Prostaglandin E2 Signaling in Concordance with Increasing Fibrosis in Ectopic Endometrium |