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验证数据展示

  • WB analysis using 66798-1-Ig (same clone as 66798-1-PBS)

    WB analysis using 66798-1-Ig (same clone as 66798-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66798-1-Ig (HPGD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • IF Staining of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    IF Staining of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using 66798-1-Ig (HPGD antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

  • IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPGD antibody included
  • IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    IHC staining of human colon using 66798-1-Ig (same clone as 66798-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66798-1-Ig (HPGD antibody) at dilution of 1:1000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HPGD antibody included
  • FC experiment of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    FC experiment of A549 using 66798-1-Ig (same clone as 66798-1-PBS)

    1X10^6 A549 cells were intracellularly stained with 0.2 ug Anti-Human HPGD (66798-1-Ig, Clone:3F1F5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.2 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 66798-1-PBS in a different storage buffer formulation.

HPGD Monoclonal antibody, PBS Only

HPGD Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 66798-1-PBS

产品说明书

CloneNo. 3F1F5

宿主/亚型

Mouse / IgG2b

种属反应性

human

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

15 PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, 3F1F5, EC:1.1.1.-, EC:1.1.1.232

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HPGD Ready-To-Use IHC Kit. HPGD primary antibody included.

产品信息

66798-1-PBS targets HPGD in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag1499

Product name: Recombinant human HPGD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC018986

Sequence: MHVNGKVALVTGAAQGIGRAFAEALLLKGAKVALVDWNLEAGVQCKAALDEQFEPQKTLFIQCDVADQQQLRDTFRKVVDHFGRLDILVNNAGVNNEKNWEKTLQINLVSVISGTYLGLDYMSKQNGGEGGIIINMSSLAGLMPVAQQPVYCASKHGIVGFTRSAALAANLMNSGVRLNAICPGFVNTAILESIEKEENMGQYIEYKDHIKDMIKYYGILDPPLIANGLITLIEDDALNGAIMKITTSKGIHFQDYDTTPFQAKTQ

种属同源性预测
宿主/亚型 Mouse / IgG2b
抗体类别 Monoclonal
产品类型 Antibody
全称 hydroxyprostaglandin dehydrogenase 15-(NAD)
别名 15 PGDH, 15-hydroxyprostaglandin dehydrogenase [NAD(+)], 15-PGDH, 3F1F5, EC:1.1.1.-, EC:1.1.1.232
计算分子量 29 kDa
观测分子量 25-28 kDa
GenBank蛋白编号BC018986
基因名称 HPGD
Gene ID (NCBI) 3248
RRIDAB_2861358
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP15428
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HPGD, also named as PGDH (15-hydroxyprostaglandin dehydrogenase), a prostaglandin-degrading enzyme that physiologically antagonizes COX-2 (PMID:15574495). HPGD inhibits in vivo proferation of colon cancer cells (PMID:24760190). HPGD has five isoforms with the molecular mass of 29, 21, 19, 16 and 15 kDa.

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