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验证数据展示

  • WB analysis using 84964-4-RR

    WB analysis using 84964-4-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 84964-4-RR (HNRNPU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IHC staining of mouse colon using 84964-4-RR

    IHC staining of mouse colon using 84964-4-RR

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84964-4-RR (HNRNPU antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HNRNPU antibody included
  • FC experiment of HepG2 using 84964-4-RR

    FC experiment of HepG2 using 84964-4-RR

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HNRNPU Recombinant antibody (84964-4-RR, Clone:242609C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HeLa using 84964-4-RR

    IF Staining of HeLa using 84964-4-RR

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPU antibody (84964-4-RR, Clone: 242609C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

HNRNPU Recombinant antibody

HNRNPU Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Cat No. 84964-4-RR

产品说明书

CloneNo. 242609C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), ELISA

别名

242609C9, C1orf199, GRIP120, hnRNP U, HNRPU

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHEK-293 cells, Jurkat cells, HeLa cells, HepG2 cells, MCF-7 cells, NIH/3T3 cells,
Positive IHC detected inmouse colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPU Ready-To-Use IHC Kit. HNRNPU primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochemistry (IHC)IHC : 1:250-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:250-1:1000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

84964-4-RR targets HNRNPU in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 HNRNPU fusion protein Ag6141 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
别名 242609C9, C1orf199, GRIP120, hnRNP U, HNRPU
计算分子量 825 aa, 91 kDa
观测分子量 120 kDa
GenBank蛋白编号BC003621
基因名称 HNRNPU
Gene ID (NCBI) 3192
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ00839
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).

实验方案

Product Specific Protocols
WB protocol for HNRNPU antibody 84964-4-RRDownload protocol
IHC protocol for HNRNPU antibody 84964-4-RRDownload protocol
IF protocol for HNRNPU antibody 84964-4-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols