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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84964-4-RR (same clone as 84964-4-PBS)

    WB analysis using 84964-4-RR (same clone as 84964-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84964-4-RR (HNRNPU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

  • IHC staining of mouse colon using 84964-4-RR (same clone as 84964-4-PBS)

    IHC staining of mouse colon using 84964-4-RR (same clone as 84964-4-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 84964-4-RR (HNRNPU antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPU antibody included
  • FC experiment of HepG2 using 84964-4-RR (same clone as 84964-4-PBS)

    FC experiment of HepG2 using 84964-4-RR (same clone as 84964-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HNRNPU Recombinant antibody (84964-4-RR, Clone:242609C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 84964-4-RR (same clone as 84964-4-PBS)

    IF Staining of HeLa using 84964-4-RR (same clone as 84964-4-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using HNRNPU antibody (84964-4-RR, Clone: 242609C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

HNRNPU Recombinant antibody, PBS Only

HNRNPU Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA
Cat No. 84964-4-PBS

产品说明书

CloneNo. 242609C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

别名

242609C9, C1orf199, GRIP120, hnRNP U, HNRPU

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HNRNPU Ready-To-Use IHC Kit. HNRNPU primary antibody included.

产品信息

84964-4-PBS targets HNRNPU in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HNRNPU fusion protein Ag6141 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
别名 242609C9, C1orf199, GRIP120, hnRNP U, HNRPU
计算分子量 825 aa, 91 kDa
观测分子量 120 kDa
GenBank蛋白编号BC003621
基因名称 HNRNPU
Gene ID (NCBI) 3192
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ00839
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).