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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 84964-4-RR (same clone as 84964-4-PBS)

    WB analysis using 84964-4-RR (same clone as 84964-4-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84964-4-RR (HNRNPU antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 84964-4-RR (same clone as 84964-4-PBS)

    FC experiment of HepG2 using 84964-4-RR (same clone as 84964-4-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.25 ug HNRNPU Recombinant antibody (84964-4-RR, Clone:242609C9) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 84964-4-PBS in a different storage buffer formulation.

HNRNPU Recombinant antibody, PBS Only

HNRNPU Uni-rAbTM Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 84964-4-PBS

产品说明书

CloneNo. 242609C9

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

Nuclear p120 ribonucleoprotein, HNRPU, hnRNP U, GRIP120, C1orf199

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy HNRNPU Ready-To-Use IHC Kit. HNRNPU primary antibody included.

产品信息

84964-4-PBS targets HNRNPU in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 HNRNPU fusion protein Ag6141 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein U (scaffold attachment factor A)
别名 Nuclear p120 ribonucleoprotein, HNRPU, hnRNP U, GRIP120, C1orf199
计算分子量 825 aa, 91 kDa
观测分子量 120 kDa
GenBank蛋白编号BC003621
基因名称 HNRNPU
Gene ID (NCBI) 3192
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ00839
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HNRNPU, also named as HNRPU, SAFA, and U21.1, is a 825 amino acid protein, which is extensively phosphorylated. HNRNPU as a component of ribonucleosomes localized in cytoplasmic mRNP granules containing untranslated mRNAs. HNRNPU is a component of the CRD-mediated complex that promotes MYC mRNA stabilization. HNRNPU Plays a role in the circadian regulation of the core clock component ARNTL/BMAL1 transcription. The calculated molecular weight of HNRNPU is 90 kDa, but the 120 kDa HNRNPU protein is an abundant nucleoplasmic phosphoprotein that can be crosslinked to pre-mRNA in intact cells by UV light (PMID: 1628625).