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CoraLite® Plus 488-conjugated HNRNPH1 Monoclonal antibody

HNRNPH1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, rat

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1A6C2

Cat No : CL488-67375

Print datasheet

Synonyms

DKFZp686A15170, hnRNP H, hnRNPH, HNRNPH1, HNRPH, HNRPH1

验证数据展示

  • IF Staining of HepG2 using CL488-67375

    IF Staining of HepG2 using CL488-67375

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CoraLite® Plus 488 HNRNPH1 antibody (CL488-67375, Clone: 1A6C2 ) at dilution of 1:100.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67375-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67375 targets HNRNPH1 in IF applications and shows reactivity with Human, mouse, rat samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, rat
Immunogen HNRNPH1 fusion protein Ag6603 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name heterogeneous nuclear ribonucleoprotein H1 (H)
Synonyms DKFZp686A15170, hnRNP H, hnRNPH, HNRNPH1, HNRPH, HNRPH1
Calculated Molecular Weight 49 kDa
Observed Molecular Weight 49-50 kDa
GenBank Accession NumberBC001348
Gene Symbol HNRNPH1
Gene ID (NCBI) 3187
RRIDAB_2919460
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP31943
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HNRNPH1 antibody CL488-67375Download protocol
Standard Protocols
Click here to view our Standard Protocols