验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, HEK-293 cells, MCF-7 cells, LNCaP cells, U2OS cells, HSC-T6 cells, 4T1 cells, Jurkat cells, NIH/3T3 cells |
| Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
67375-1-Ig targets HNRNPH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human |
| 免疫原 |
CatNo: Ag6603 Product name: Recombinant human HNRNPH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 104-449 aa of BC001348 Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA 种属同源性预测 |
| 宿主/亚型 | Mouse / IgG1 |
| 抗体类别 | Monoclonal |
| 产品类型 | Antibody |
| 全称 | heterogeneous nuclear ribonucleoprotein H1 (H) |
| 别名 | HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed |
| 计算分子量 | 49 kDa |
| 观测分子量 | 49-50 kDa |
| GenBank蛋白编号 | BC001348 |
| 基因名称 | HNRNPH1 |
| Gene ID (NCBI) | 3187 |
| RRID | AB_2882623 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Protein G purification |
| UNIPROT ID | P31943 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for HNRNPH1 antibody 67375-1-Ig | Download protocol |
| IHC protocol for HNRNPH1 antibody 67375-1-Ig | Download protocol |
| WB protocol for HNRNPH1 antibody 67375-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Front Oncol MS0621, a novel small-molecule modulator of Ewing sarcoma chromatin accessibility, interacts with an RNA-associated macromolecular complex and influences RNA splicing | ||
J Cell Physiol MACC1 enhances an oncogenic RNA splicing of IRAK1 through interacting with HNRNPH1 in lung adenocarcinoma | ||
Int J Biol Macromol Japanese encephalitis virus NS3 captures the protein translation element by interacting with HNRNPH1 to promote viral replication
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