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验证数据展示

  • WB analysis using 67375-1-Ig (same clone as 67375-1-PBS)

    WB analysis using 67375-1-Ig (same clone as 67375-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67375-1-Ig (same clone as 67375-1-PBS)

    IHC staining of human breast cancer using 67375-1-Ig (same clone as 67375-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67375-1-Ig (HNRNPH1 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HNRNPH1 antibody included
  • IF Staining of HepG2 using 67375-1-Ig (same clone as 67375-1-PBS)

    IF Staining of HepG2 using 67375-1-Ig (same clone as 67375-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67375-1-Ig (HNRNPH1 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67375-1-PBS in a different storage buffer formulation.

HNRNPH1 Monoclonal antibody, PBS Only

HNRNPH1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67375-1-PBS

产品说明书

CloneNo. 1A6C2

宿主/亚型

Mouse / IgG1

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HNRNPH1 Ready-To-Use IHC Kit. HNRNPH1 primary antibody included.

产品信息

67375-1-PBS targets HNRNPH1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6603

Product name: Recombinant human HNRNPH1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 104-449 aa of BC001348

Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein H1 (H)
别名 HNRPH1, HNRPH, hnRNPH, hnRNP H, Heterogeneous nuclear ribonucleoprotein H, N-terminally processed
计算分子量 49 kDa
观测分子量 49-50 kDa
GenBank蛋白编号BC001348
基因名称 HNRNPH1
Gene ID (NCBI) 3187
RRIDAB_2882623
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDP31943
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

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