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验证数据展示

  • WB analysis using 88017-1-RR (same clone as 88017-1-PBS)

    WB analysis using 88017-1-RR (same clone as 88017-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 88017-1-RR (HNRNPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 88017-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 88017-1-RR (same clone as 88017-1-PBS)

    IF Staining of HepG2 using 88017-1-RR (same clone as 88017-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPH1 antibody (88017-1-RR, Clone: 260181B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 88017-1-PBS in a different storage buffer formulation.

  • Cytometric bead array sample test of MP03314-1

    Cytometric bead array sample test of MP03314-1

    The mean HNRNPH1 concentration was determined to be 21.9 ng/mL in HepG2 cell extract based on a 1.2 mg/mL extract load, 0.3 ng/mL in Y79 cell extract based on a 1.1 mg/mL extract load.

  • Cytometric bead array standard curve of MP03314-1

    Cytometric bead array standard curve of MP03314-1

    Cytometric bead array standard curve of MP03314-1, HNRNPH1 Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 88017-1-PBS. Detection antibody: 88017-2-PBS. Standard: Ag6506. Range: 0.156-20 ng/mL.

  • Affinity of 88017-1-RR

    Affinity of 88017-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88017-1-RR against Human HNRNPH1 were performed. The affinity constant is 0.905 nM.

HNRNPH1 Recombinant monoclonal antibody, PBS Only (Capture)

HNRNPH1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Cytometric bead array, Indirect ELISA
Cat No. 88017-1-PBS

产品说明书

CloneNo. 260181B8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, Cytometric bead array, Indirect ELISA

别名

Heterogeneous nuclear ribonucleoprotein H, N-terminally processed, hnRNP H, hnRNPH, HNRPH, HNRPH1

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HNRNPH1 Ready-To-Use IHC Kit. HNRNPH1 primary antibody included.

产品信息

88017-1-PBS targets HNRNPH1 as part of a matched antibody pair:

MP03314-1: 88017-1-PBS capture and 88017-2-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

经测试应用 WB, IF/ICC, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6506

Product name: Recombinant human HNRNPH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 104-449 aa of BC001348

Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein H1 (H)
别名 Heterogeneous nuclear ribonucleoprotein H, N-terminally processed, hnRNP H, hnRNPH, HNRPH, HNRPH1
计算分子量 49 kDa
观测分子量 49-50 kDa
GenBank蛋白编号BC001348
基因名称 HNRNPH1
Gene ID (NCBI) 3187
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31943
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

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