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验证数据展示

  • WB analysis using 88017-1-RR

    WB analysis using 88017-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 88017-1-RR (HNRNPH1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of HepG2 using 88017-1-RR

    IF Staining of HepG2 using 88017-1-RR

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using HNRNPH1 antibody (88017-1-RR, Clone: 260181B8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity of 88017-1-RR

    Affinity of 88017-1-RR

    Biolayer interferometry (BLI) kinetic assays of 88017-1-RR against Human HNRNPH1 were performed. The affinity constant is 0.905 nM.

HNRNPH1 Recombinant monoclonal antibody

HNRNPH1 Uni-rAb® Recombinant Antibody for WB, IF/ICC, ELISA
Cat No. 88017-1-RR

产品说明书

CloneNo. 260181B8

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IF/ICC, ELISA

别名

Heterogeneous nuclear ribonucleoprotein H, N-terminally processed, hnRNP H, hnRNPH, HNRPH, HNRPH1

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, HepG2 cells, HEK-293T cells, 4T1 cells, NIH/3T3 cells, HSC-T6 cells, mouse brain tissue
Positive IF/ICC detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy HNRNPH1 Ready-To-Use IHC Kit. HNRNPH1 primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

88017-1-RR targets HNRNPH1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IF/ICC, ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag6506

Product name: Recombinant human HNRNPH1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 104-449 aa of BC001348

Sequence: SPDTANDGFVRLRGLPFGCSKEEIVQFFSGLEIVPNGITLPVDFQGRSTGEAFVQFASQEIAEKALKKHKERIGHRYIEIFKSSRAEVRTHYDPPRKLMAMQRPGPYDRPGAGRGYNSIGRGAGFERMRRGAYGGGYGGYDDYNGYNDGYGFGSDRFGRDLNYCFSGMSDHRYGDGGSTFQSTTGHCVHMRGLPYRATENDIYNFFSPLNPVRVHIEIGPDGRVTGEADVEFATHEDAVAAMSKDKANMQHRYVELFLNSTAGASGGAYEHRYVELFLNSTAGASGGAYGSQMMGGMGLSNQSSYGGPASQQLSGGYGGGYGGQSSMSGYDQVLQENSSDFQSNIA

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 heterogeneous nuclear ribonucleoprotein H1 (H)
别名 Heterogeneous nuclear ribonucleoprotein H, N-terminally processed, hnRNP H, hnRNPH, HNRPH, HNRPH1
计算分子量 49 kDa
观测分子量 49-50 kDa
GenBank蛋白编号BC001348
基因名称 HNRNPH1
Gene ID (NCBI) 3187
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP31943
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

The heterogeneous nuclear ribonucleoprotein (hnRNP) complexes which provide the substrate for the processing events that pre-mRNAs undergo before becoming functional, translatable mRNAs in the cytoplasm. HNRNPH1 is one component of hnRNP complex and mediates pre-mRNA alternative splicing regulation [PMID:11003644]. Together with CUGBP1, it inhibits IR pre-mRNA exon 11 inclusion in myoblast [PMID: 16946708].

实验方案

Product Specific Protocols
IF protocol for HNRNPH1 antibody 88017-1-RRDownload protocol
WB protocol for HNRNPH1 antibody 88017-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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