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验证数据展示

  • WB analysis using 83103-1-RR

    WB analysis using 83103-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • IHC staining of human tonsillitis using 83103-1-RR

    IHC staining of human tonsillitis using 83103-1-RR

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of Raji using 83103-1-RR

    IF Staining of Raji using 83103-1-RR

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

  • Affinity and Kinetic Characterization of 83103-1-RR

    Affinity and Kinetic Characterization of 83103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

HLA-DRB4 Recombinant monoclonal antibody

HLA-DRB4 Recombinant Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 83103-1-RR

产品说明书

CloneNo. 230352F10

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, IF/ICC, ELISA

别名

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inRaji cells, Ramos cells, Daudi cells, pig spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inRaji cells

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:8000
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

83103-1-RR targets HLA-DRB4 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag8492

Product name: Recombinant human HLA-DRB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC005312

Sequence: MVCLKLPGGSCMAALTVTLTVLSSPLALAGDTQPRFLEQAKCECHFLNGTERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSMMSPLTVQWSARSESAQSKMLSGVGGFVLGLLFLGTGLFIYFRNQKGHSGLQPTGLLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 major histocompatibility complex, class II, DR beta 4
别名 MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10
计算分子量 266 aa, 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC005312
基因名称 HLA-DRB4
Gene ID (NCBI) 3126
RRIDAB_3670816
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13762
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

实验方案

Product Specific Protocols
IF protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
IHC protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
WB protocol for HLA-DRB4 antibody 83103-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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