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验证数据展示

  • WB analysis using 83103-1-RR (same clone as 83103-1-PBS)

    WB analysis using 83103-1-RR (same clone as 83103-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • IHC staining of human tonsillitis using 83103-1-RR (same clone as 83103-1-PBS)

    IHC staining of human tonsillitis using 83103-1-RR (same clone as 83103-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83103-1-RR (HLA-DRB4 antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • IF Staining of Raji using 83103-1-RR (same clone as 83103-1-PBS)

    IF Staining of Raji using 83103-1-RR (same clone as 83103-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed Raji cells using HLA-DRB4 antibody (83103-1-RR, Clone: 230352F10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83103-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 83103-1-RR

    Affinity and Kinetic Characterization of 83103-1-RR

    Biolayer interferometry (BLl) kinetic assays of 83103-1-RR against Human HLA-DRB4 were performed. The affinity constant is 0.623 nM.

HLA-DRB4 Recombinant monoclonal antibody, PBS Only

HLA-DRB4 Uni-rAb® Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 83103-1-PBS

产品说明书

CloneNo. 230352F10

宿主/亚型

Rabbit / IgG

种属反应性

human, pig

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83103-1-PBS targets HLA-DRB4 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, pig samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human, pig
免疫原

CatNo: Ag8492

Product name: Recombinant human HLA-DRB4 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-266 aa of BC005312

Sequence: MVCLKLPGGSCMAALTVTLTVLSSPLALAGDTQPRFLEQAKCECHFLNGTERVWNLIRYIYNQEEYARYNSDLGEYQAVTELGRPDAEYWNSQKDLLERRRAEVDTYCRYNYGVVESFTVQRRVQPKVTVYPSKTQPLQHHNLLVCSVNGFYPGSIEVRWFRNGQEEKAGVVSTGLIQNGDWTFQTLVMLETVPRSGEVYTCQVEHPSMMSPLTVQWSARSESAQSKMLSGVGGFVLGLLFLGTGLFIYFRNQKGHSGLQPTGLLS

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 major histocompatibility complex, class II, DR beta 4
别名 MHC class II antigen DRB4, HLA DRB4, HLA DR4B, 230352F10
计算分子量 266 aa, 30 kDa
观测分子量30 kDa
GenBank蛋白编号BC005312
基因名称 HLA-DRB4
Gene ID (NCBI) 3126
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP13762
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C
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