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验证数据展示

  • Dot Blot experiment of peptide using 83041-1-RR (same clone as 83041-1-PBS)

    Dot Blot experiment of peptide using 83041-1-RR (same clone as 83041-1-PBS)

    Dot blot analysis was used to confirm the specificity of Acetyl-Histone H2A (Lys9) antibody. Acetylated peptides were spotted onto NC and probed with antibody at 1 µg/ml.The amount of peptide (μg/mL) spotted is indicated next to each row.Column 1: H2AEK9Ac. Column 2: Unmodified H2AEK9. Column 3: H2AEK5Ac. Column 4: Unmodified H2AEK5. Column 5: H2AEK13Ac. Column 6: Unmodified H2AEK13. Column 7: H2AEK15Ac. Column 8: Unmodified H2AEK15. Column 9: Blank(PBS). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • ChIP experiment of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    ChIP experiment of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    Chromatin was prepared from HeLa cells, cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 15 µg of cross-linked chromatin, 5 µg of Acetyl-Histone H2A (Lys9) (83041-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR. This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • WB analysis of NIH/3T3 using 83041-1-RR (same clone as 83041-1-PBS)

    WB analysis of NIH/3T3 using 83041-1-RR (same clone as 83041-1-PBS)

    Trichostatin A treated NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with Beta Actin Monoclonal antibody (66009-1-Ig) as loading control. This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 83041-1-RR (same clone as 83041-1-PBS)

    IHC staining of mouse lung using 83041-1-RR (same clone as 83041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse lung using 83041-1-RR (same clone as 83041-1-PBS)

    IHC staining of mouse lung using 83041-1-RR (same clone as 83041-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 83041-1-RR (Acetyl-Histone H2A (Lys9) antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1) at dilution of 1:150 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    IF Staining of HeLa using 83041-1-RR (same clone as 83041-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Acetyl-Histone H2A (Lys9) antibody (83041-1-RR, Clone: 230349B1 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 83041-1-PBS in a different storage buffer formulation.

Acetyl-Histone H2A (Lys9) Recombinant antibody, PBS Only (Capture)

Acetyl-Histone H2A (Lys9) Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, ChIP, Dot Blot, Cytometric bead array, Indirect ELISA
Cat No. 83041-1-PBS

产品说明书

CloneNo. 230349B1

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF/ICC, ChIP, Dot Blot, Cytometric bead array, Indirect ELISA

别名

Ac Histone H2A, Ac Histone H2A (Lys9), Ac Histone H2A Lys9, Ac Histone H2A-Lys9, Acetyl Histone

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

83041-1-PBS targets Acetyl-Histone H2A (Lys9) in WB, IHC, IF/ICC, ChIP, Dot Blot, Cytometric bead array, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF/ICC, ChIP, Dot Blot, Cytometric bead array, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 histone cluster 1, H2ae
别名 Ac Histone H2A, Ac Histone H2A (Lys9), Ac Histone H2A Lys9, Ac Histone H2A-Lys9, Acetyl Histone
计算分子量 14 kDa
观测分子量14 kDa
GenBank蛋白编号BC093836
基因名称 HIST1H2AE
Gene ID (NCBI) 3012
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDP04908
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

Histone H2A is a core component of nucleosome. Histone variants contribute to chromatin complexity by creating specialized nucleosomes. Within nucleosomes, either one canonical H2A or both of them can be exchanged with a particular variant (heterotypic and homotypic nucleosomes, respectively), and such changes can have profound influences on nucleosome stability and biological outcome.