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验证数据展示

  • WB analysis of mouse brain using 13015-1-AP (same clone as 13015-1-PBS)

    WB analysis of mouse brain using 13015-1-AP (same clone as 13015-1-PBS)

    mouse brain tissue were subjected to SDS PAGE followed by western blot with 13015-1-AP (DBNL antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13015-1-AP (same clone as 13015-1-PBS)

    IHC staining of human breast cancer using 13015-1-AP (same clone as 13015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 13015-1-AP (same clone as 13015-1-PBS)

    IHC staining of human breast cancer using 13015-1-AP (same clone as 13015-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 13015-1-AP (DBNL antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

  • IP experiment of mouse brain using 13015-1-AP (same clone as 13015-1-PBS)

    IP experiment of mouse brain using 13015-1-AP (same clone as 13015-1-PBS)

    IP result of anti-HIP55 (IP:13015-1-AP, 4ug; Detection:13015-1-AP 1:1000) with mouse brain tissue lysate 2640ug. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

  • IF Staining of HepG2 using 13015-1-AP (same clone as 13015-1-PBS)

    IF Staining of HepG2 using 13015-1-AP (same clone as 13015-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using HIP55 antibody (13015-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

  • FC experiment of HepG2 using 13015-1-AP (same clone as 13015-1-PBS)

    FC experiment of HepG2 using 13015-1-AP (same clone as 13015-1-PBS)

    1x10^6 HepG2 cells were intracellularly stained with 0.4 ug HIP55 Polyclonal antibody (13015-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 13015-1-PBS in a different storage buffer formulation.

  • Featured Product
  • KD/KO Validated

HIP55 Polyclonal antibody, PBS Only

HIP55 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA
Cat No. 13015-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA

别名

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

13015-1-PBS targets HIP55 in WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

经测试应用 WB, IHC, IF/ICC, FC (Intra), IP, Indirect ELISA Application Description
经测试反应性 human, mouse, rat
免疫原

CatNo: Ag4078

Product name: Recombinant human DBNL protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 131-430 aa of BC031687

Sequence: KVAKASGANYSFHKESGRFQDVGPQAPVGSVYQKTNAVSEIKRVGKDSFWAKAEKEEENRRLEEKRRAEEAQRQLEQERRERELREAARREQRYQEQGGEASPQRTWEQQQEVVSRNRNEQESAVHPREIFKQKERAMSTTSISSPQPGKLRSPFLQKQLTQPETHFGREPAAAISRPRADLPAEEPAPSTPPCLVQAEEEAVYEEPPEQETFYEQPPLVQQQGAGSEHIDHHIQGQGLSGQGLCARALYDYQAADDTEISFDPENLITGIEVIDEGWWRGYGPDGHFGMFPANYVELIE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 drebrin-like
别名
计算分子量 430 aa, 48 kDa
观测分子量 55 kDa
GenBank蛋白编号BC031687
基因名称 HIP55
Gene ID (NCBI) 28988
RRIDAB_2230306
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ9UJU6
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HIP55, also known as DBNL, ABP1, SH3P7, belongs to the ABP1 family. HIP55 plays a role in the reorganization of the actin cytoskeleton, formation of cell projections, such as neurites, in neuron morphogenesis and synapse formation via its interaction with WASL and COBL. HIP55 acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes (PMID: 14729663). The N terminus of HIP55 contains a putative actin-binding domain found in drebrins, which are involved in brain development, and the C terminus contains an SH3 domain. Expect a band 55 kDa in size corresponding to HIP55 by western blotting.

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