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HIP55 Polyclonal antibody
HIP55 Polyclonal Antibody for IF/ICC, IHC, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
IF/ICC, IHC, IP, WB, ELISA
Conjugate
Unconjugated
验证数据展示
经过测试的应用
Positive WB detected in | mouse brain tissue, HeLa cells, human heart tissue, Jurkat cells, K-562 cells, mouse heart tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | human breast cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | HepG2 cells |
推荐稀释比
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:20-1:200 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
产品信息
13015-1-AP targets HIP55 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Applications | IF/ICC, IHC, IP, WB, ELISA |
Cited Applications | WB, IP, IF |
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse |
Immunogen | HIP55 fusion protein Ag4078 种属同源性预测 |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Full Name | drebrin-like |
Synonyms | ABP1, Cervical SH3P7, CMAP, DBNL, Drebrin F, drebrin like, Drebrin like protein, HIP 55, SH3P7 |
Calculated Molecular Weight | 430 aa, 48 kDa |
Observed Molecular Weight | 55 kDa |
GenBank Accession Number | BC031687 |
Gene Symbol | DBNL |
Gene ID (NCBI) | 28988 |
RRID | AB_2230306 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
UNIPROT ID | Q9UJU6 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
实验方案
Product Specific Protocols | |
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WB protocol for HIP55 antibody 13015-1-AP | Download protocol |
IHC protocol for HIP55 antibody 13015-1-AP | Download protocol |
IF protocol for HIP55 antibody 13015-1-AP | Download protocol |
IP protocol for HIP55 antibody 13015-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
发表文章
Species | Application | Title |
---|---|---|
Acta Pharmacol Sin The multifunctional adaptor protein HIP-55 couples Smad7 to accelerate TGF-β type I receptor degradation.
| ||
J Neurosci Drebrin-like (Dbnl) controls neuronal migration via regulating N-cadherin expression in the developing cerebral cortex. | ||
J Cell Sci VEGF-A stimulates podosome-mediated collagen-IV proteolysis in microvascular endothelial cells. | ||
J Microbiol Effect of Salmonella treatment on an implanted tumor (CT26) in a mouse model. | ||