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CoraLite®594-conjugated HIP1 Monoclonal antibody

HIP1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, mouse, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

3D3G3

Cat No : CL594-67163

Print datasheet

Synonyms

HIP 1, HIP I, HIP1, ILWEQ

验证数据展示

  • IF Staining of A549 using CL594-67163

    IF Staining of A549 using CL594-67163

    Immunofluorescent analysis of (4% PFA) fixed A549 cells using CoraLite®594 HIP1 antibody (CL594-67163, Clone: 3D3G3 ) at dilution of 1:200, CL488-Phalloidin (green).

经过测试的应用

Positive IF detected inA549 cells
Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67163-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-67163 targets HIP1 in IF applications and shows reactivity with Human, mouse, pig samples.

Tested Applications IF/ICC
Tested Reactivity Human, mouse, pig
Immunogen HIP1 fusion protein Ag17982 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name huntingtin interacting protein 1
Synonyms HIP 1, HIP I, HIP1, ILWEQ
Calculated Molecular Weight 1037 aa, 116 kDa
Observed Molecular Weight 110-120 kDa
GenBank Accession NumberBC110545
Gene Symbol HIP1
Gene ID (NCBI) 3092
RRIDAB_2920092
Conjugate CoraLite®594 Fluorescent Dye
Excitation/Emission Maxima Wavelengths588 nm / 604 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDO00291
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

实验方案

Product Specific Protocols
IF protocol for CL594 HIP1 antibody CL594-67163Download protocol
Standard Protocols
Click here to view our Standard Protocols