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验证数据展示

  • WB analysis using 67163-1-Ig

    WB analysis using 67163-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67163-1-Ig (HIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67163-1-Ig

    WB analysis of HeLa using 67163-1-Ig

    WB result of HIP1 antibody (67163-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-HIP1 transfected HeLa cells.

  • IHC staining of human colon cancer using 67163-1-Ig

    IHC staining of human colon cancer using 67163-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IHC staining of human colon cancer using 67163-1-Ig

    IHC staining of human colon cancer using 67163-1-Ig

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67163-1-Ig (HIP1 antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HIP1 antibody included
  • IF Staining of human colon cancer using 67163-1-Ig

    IF Staining of human colon cancer using 67163-1-Ig

    Immunofluorescent analysis of (4% PFA) fixed human colon cancer tissue using HIP1 antibody (67163-1-Ig, Clone: 3D3G3 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L).

  • Featured Product
  • KD/KO验证

HIP1 Monoclonal antibody

HIP1 Monoclonal Antibody for WB, IHC, IF-P, ELISA
Cat No. 67163-1-Ig

产品说明书

CloneNo. 3D3G3

宿主/亚型

Mouse / IgG1

种属反应性

Human, mouse, pig

应用

WB, IHC, IF-P, ELISA

别名

HIP 1, HIP I, HIP1, ILWEQ

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, pig brain tissue, mouse brain tissue, A549 cells, PC-3 cells
Positive IHC detected inhuman colon cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman colon cancer tissue
Planning an IHC experiment? We recommend our IHCeasy HIP1 Ready-To-Use IHC Kit. HIP1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:150-1:600
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67163-1-Ig targets HIP1 in WB, IHC, IF-P, ELISA applications and shows reactivity with Human, mouse, pig samples.

经测试应用 WB, IHC, IF-P, ELISA Application Description
经测试反应性 Human, mouse, pig
免疫原

CatNo: Ag17982

Product name: Recombinant human HIP1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 435-764 aa of BC110545

Sequence: AELDELRRQREDTEKAQRSLSEIERKAQANEQRYSKLKEKYSELVQNHADLLRKNAEVTKQVSMARQAQVDLEREKKELEDSLERISDQGQRKTQEQLEVLESLKQELATSQRELQVLQGSLETSAQSEANWAAEFAELEKERDSLVSGAAHREEELSALRKELQDTQLKLASTEESMCQLAKDQRKMLLVGSRKAAEQVIQDALNQLEEPPLISCAGSADHLLSTVTSISSCIEQLEKSWSQYLACPEDISGLLHSITLLAHLTSDAIAHGATTCLRAPPEPADSLTEACKQYGRETLAYLASLEEEGSLENADSTAMRNCLSKIKAIG

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 huntingtin interacting protein 1
别名 HIP 1, HIP I, HIP1, ILWEQ
计算分子量 1037 aa, 116 kDa
观测分子量 110-120 kDa
GenBank蛋白编号BC110545
基因名称 HIP1
Gene ID (NCBI) 3092
RRIDAB_2882459
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDO00291
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HIP1 (huntingtin interacting protein-1) is a endocytic protein that plays a role in clathrin-mediated endocytosis and trafficking. HIP1 can interact with huntingtin (HTT) protein whose mutations cause Huntington's disease (HD), thus gets involved in neurodegeneration. By stabilizing receptor tyrosine kinases following ligand-induced endocytosis, HIP1 may act to promote cell survival. HIP1 is overexpressed in a variety of human cancers and may be a potential predictor for cancer progression and prognosis.

实验方案

Product Specific Protocols
IF protocol for HIP1 antibody 67163-1-IgDownload protocol
IHC protocol for HIP1 antibody 67163-1-IgDownload protocol
WB protocol for HIP1 antibody 67163-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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