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验证数据展示

  • IF Staining of HeLa using CL488-67583

    IF Staining of HeLa using CL488-67583

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CoraLite® Plus 488 HINT1 antibody (CL488-67583, Clone: 1G3E4 ) at dilution of 1:200.

CoraLite® Plus 488-conjugated HINT1 Monoclonal antibody

HINT1 Monoclonal Antibody for IF/ICC
Cat No. CL488-67583

产品说明书

CloneNo. 1G3E4

宿主/亚型

Mouse / IgG2a

种属反应性

Human

应用

IF/ICC

别名

FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HINT1 Ready-To-Use IHC Kit. HINT1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67583-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67583 targets HINT1 in IF/ICC applications and shows reactivity with Human samples.

经测试应用 IF/ICC Application Description
经测试反应性 Human
免疫原

CatNo: Ag16662

Product name: Recombinant human HINT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-126 aa of BC007090

Sequence: MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 histidine triad nucleotide binding protein 1
别名 FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC007090
基因名称 HINT1
Gene ID (NCBI) 3094
RRIDAB_3084361
偶联类型 CoraLite® Plus 488 Fluorescent Dye
最大激发/发射波长493 nm / 522 nm
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49773
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 HINT1 antibody CL488-67583Download protocol
Standard Protocols
Click here to view our Standard Protocols
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