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HINT1 Monoclonal antibody

HINT1 Monoclonal Antibody for IF/ICC, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF/ICC, IHC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G3E4

Cat No : 67583-1-Ig

Print datasheet

Synonyms

FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1

验证数据展示

  • IHC staining of human breast cancer using 67583-1-Ig

    IHC staining of human breast cancer using 67583-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • WB analysis using 67583-1-Ig

    WB analysis using 67583-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67583-1-Ig (HINT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 67583-1-Ig

    IHC staining of human breast cancer using 67583-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of HeLa using 67583-1-Ig

    IF Staining of HeLa using 67583-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HINT1 antibody (67583-1-Ig, Clone: 1G3E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

67583-1-Ig targets HINT1 in WB, IF, IHC, ELISA applications and shows reactivity with Human samples.

Tested Applications IF/ICC, IHC, WB, ELISA
Tested Reactivity Human
Immunogen HINT1 fusion protein Ag16662 种属同源性预测
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Full Name histidine triad nucleotide binding protein 1
Synonyms FLJ30414, FLJ32340, HINT, HINT1, PKCI 1, PKCI1, PRKCNH1, Protein kinase C inhibitor 1
Calculated Molecular Weight 14 kDa
Observed Molecular Weight 14 kDa
GenBank Accession NumberBC007090
Gene Symbol HINT1
Gene ID (NCBI) 3094
RRIDAB_2882793
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP49773
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.

实验方案

Product Specific Protocols
WB protocol for HINT1 antibody 67583-1-IgDownload protocol
IHC protocol for HINT1 antibody 67583-1-IgDownload protocol
IF protocol for HINT1 antibody 67583-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols