• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 67583-1-Ig (same clone as 67583-1-PBS)

    WB analysis using 67583-1-Ig (same clone as 67583-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 67583-1-Ig (HINT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

  • WB analysis of HeLa using 67583-1-Ig (same clone as 67583-1-PBS)

    WB analysis of HeLa using 67583-1-Ig (same clone as 67583-1-PBS)

    WB result of HINT1 antibody (67583-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-HINT1 transfected HeLa cells. This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 67583-1-Ig (same clone as 67583-1-PBS)

    IF Staining of HeLa using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HINT1 antibody (67583-1-Ig, Clone: 1G3E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

  • IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HINT1 antibody included
  • IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    IHC staining of human breast cancer using 67583-1-Ig (same clone as 67583-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67583-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HINT1 antibody included
  • Featured Product
  • KD/KO验证

HINT1 Monoclonal antibody, PBS Only

HINT1 Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 67583-1-PBS

产品说明书

CloneNo. 1G3E4

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

1G3E4, Adenosine 5'-monophosphoramidase HINT1, Desumoylating isopeptidase HINT1, EC:3.4.22.-, EC:3.9.1.-

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

点击咨询售前客服

点击咨询售后客服

Planning an IHC experiment? We recommend our IHCeasy HINT1 Ready-To-Use IHC Kit. HINT1 primary antibody included.

产品信息

67583-1-PBS targets HINT1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag16662

Product name: Recombinant human HINT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-126 aa of BC007090

Sequence: MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 histidine triad nucleotide binding protein 1
别名 1G3E4, Adenosine 5'-monophosphoramidase HINT1, Desumoylating isopeptidase HINT1, EC:3.4.22.-, EC:3.9.1.-
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC007090
基因名称 HINT1
Gene ID (NCBI) 3094
RRIDAB_2882793
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49773
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.

||
New chat Able
正在加载，请稍候...