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验证数据展示

  • WB analysis using 67583-1-Ig

    WB analysis using 67583-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67583-1-Ig (HINT1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  • WB analysis of HeLa using 67583-1-Ig

    WB analysis of HeLa using 67583-1-Ig

    WB result of HINT1 antibody (67583-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-HINT1 transfected HeLa cells.

  • IF Staining of HeLa using 67583-1-Ig

    IF Staining of HeLa using 67583-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using HINT1 antibody (67583-1-Ig, Clone: 1G3E4 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IHC staining of human breast cancer using 67583-1-Ig

    IHC staining of human breast cancer using 67583-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HINT1 antibody included
  • IHC staining of human breast cancer using 67583-1-Ig

    IHC staining of human breast cancer using 67583-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67583-1-Ig (HINT1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HINT1 antibody included
  • Featured Product
  • KD/KO验证

HINT1 Monoclonal antibody

HINT1 Monoclonal Antibody for WB, IHC, IF/ICC, ELISA
Cat No. 67583-1-Ig

产品说明书

CloneNo. 1G3E4
引用文献(1)

宿主/亚型

Mouse / IgG2a

种属反应性

human and More (1)

应用

WB, IHC, IF/ICC, ELISA

别名

1G3E4, Adenosine 5'-monophosphoramidase HINT1, Desumoylating isopeptidase HINT1, EC:3.4.22.-, EC:3.9.1.-

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inLNCaP cells, HeLa cells, HepG2 cells, Jurkat cells, K-562 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy HINT1 Ready-To-Use IHC Kit. HINT1 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

发表文章中的应用

WBSee 1 publications below

产品信息

67583-1-Ig targets HINT1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, ELISA Application Description
文献引用应用WB
经测试反应性 human
文献引用反应性mouse
免疫原

CatNo: Ag16662

Product name: Recombinant human HINT1 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 1-126 aa of BC007090

Sequence: MADEIAKAQVARPGGDTIFGKIIRKEIPAKIIFEDDRCLAFHDISPQAPTHFLVIPKKHISQISVAEDDDESLLGHLMIVGKKCAADLGLNKGYRMVVNEGSDGGQSVYHVHLHVLGGRQMHWPPG

种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 histidine triad nucleotide binding protein 1
别名 1G3E4, Adenosine 5'-monophosphoramidase HINT1, Desumoylating isopeptidase HINT1, EC:3.4.22.-, EC:3.9.1.-
计算分子量 14 kDa
观测分子量 14 kDa
GenBank蛋白编号BC007090
基因名称 HINT1
Gene ID (NCBI) 3094
RRIDAB_2882793
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP49773
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

HINT1(Histidine triad nucleotide-binding protein 1) is also named as HINT, PKCI1, PRKCNH1,which is a member of the histidine triad (HIT) family, highly conserved in diverse species and ubiquitously expressed in mammalian tissues.It hydrolyzes adenosine 5'-monophosphoramidate substrates such as AMP-morpholidate, AMP-N-alanine methyl ester, AMP-alpha-acetyl lysine methyl ester and AMP-NH2.

实验方案

Product Specific Protocols
IF protocol for HINT1 antibody 67583-1-IgDownload protocol
IHC protocol for HINT1 antibody 67583-1-IgDownload protocol
WB protocol for HINT1 antibody 67583-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Cell Death Dis

Histidine re-sensitizes pediatric acute lymphoblastic leukemia to 6-mercaptopurine through tetrahydrofolate consumption and SIRT5-mediated desuccinylation

Authors - Na Dong
View Article
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