验证数据展示
经过测试的应用
| Positive FC detected in | NIH/3T3 cells |
For other applications, we recommend the unconjugated version of this antibody, 98153-1-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL647-98153 targets HER2/ErbB2 in FC applications and shows reactivity with mouse samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | mouse |
| 免疫原 | HER2/ErbB2 fusion protein Eg0803 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | v-erb-b2 erythroblastic leukemia viral oncogene homolog 2, neuro/glioblastoma derived oncogene homolog (avian) |
| 别名 | 241366E6, CD340, EC:2.7.10.1, Erbb2, Her2 |
| 计算分子量 | 139 kDa |
| GenBank蛋白编号 | NM_001003817.1 |
| 基因名称 | Erbb2 |
| Gene ID (NCBI) | 13866 |
| 偶联类型 | CoraLite® Plus 647 Fluorescent Dye |
| 最大激发/发射波长 | 654 nm / 674 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P70424 |
| 储存缓冲液 | PBS with 0.09% sodium azide , pH 7.3 |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
HER2, also known as ErbB2, Neu, and CD340, is a 185-kDa transmembrane glycoprotein member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. It has no ligand-binding domain and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signaling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase.
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for CL Plus 647 HER2/ErbB2 antibody CL647-98153 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |