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验证数据展示

  • IF Staining of SKOV-3 using CL594-66557

    IF Staining of SKOV-3 using CL594-66557

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using CoraLite®594 HE4 antibody (CL594-66557, Clone: 5B2D9 ) at dilution of 1:200.

  • FC experiment of OVCAR-3 using CL594-66557

    FC experiment of OVCAR-3 using CL594-66557

    1x10^6 OVCAR-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated HE4 Monoclonal antibody (CL594-66557, Clone:5B2D9)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (1F8D3) (CL594-66360-1, Clone: 1F8D3) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • FC experiment of SKOV-3 using CL594-66557

    FC experiment of SKOV-3 using CL594-66557

    1x10^6 SKOV-3 cells were intracellularly stained with 0.4 ug CoraLite®594-conjugated HE4 Monoclonal antibody (CL594-66557, Clone:5B2D9)(red), or 0.4 ug CoraLite®594 Mouse IgG1 Isotype Control (MOPC-21) (CL594-65124, Clone: MOPC-21) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

CoraLite®594-conjugated HE4 Monoclonal antibody

HE4 Monoclonal Antibody for IF/ICC
Cat No. CL594-66557

产品说明书

CloneNo. 5B2D9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC

别名

WFDC2, 5B2D9, Epididymal secretory protein E4, Major epididymis-specific protein E4, WAP four disulfide core domain protein 2

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inSKOV-3 cells
Positive FC (Intra) detected inSKOV-3 cells, OVCAR-3 cells
Planning an IHC experiment? We recommend our IHCeasy HE4 Ready-To-Use IHC Kit. HE4 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66557-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66557 targets HE4 in IF/ICC applications and shows reactivity with human samples.

经测试应用 IF/ICC Application Description
经测试反应性 human
免疫原

CatNo: Ag5936

Product name: Recombinant human HE4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-124 aa of BC046106

Sequence: GFTLVSGTGAEKTGVCPELQADQNCTQECVSDSECADNLKCCSAGCATFCSLPNDKEGSCPQVNINFPQLGLCRDQCQVDSQCPGQMKCCRNGCGKVSCVTPNF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 WAP four-disulfide core domain 2
别名 WFDC2, 5B2D9, Epididymal secretory protein E4, Major epididymis-specific protein E4, WAP four disulfide core domain protein 2
计算分子量 13 kDa
GenBank蛋白编号BC046106
基因名称 HE4
Gene ID (NCBI) 10406
RRIDAB_2920004
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14508
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

实验方案

Product Specific Protocols
FC protocol for CL594 HE4 antibody CL594-66557Download protocol
IF protocol for CL594 HE4 antibody CL594-66557Download protocol
Standard Protocols
Click here to view our Standard Protocols
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