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验证数据展示

  • IHC staining of human ovary tumor using 66557-1-Ig

    IHC staining of human ovary tumor using 66557-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HE4 antibody included
  • IHC staining of human ovary tumor using 66557-1-Ig

    IHC staining of human ovary tumor using 66557-1-Ig

    Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66557-1-Ig (HE4 antibody) at dilution of 1:5000 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

    Ready-to-use IHC kits available with HE4 antibody included
  • IF Staining of SKOV-3 using 66557-1-Ig

    IF Staining of SKOV-3 using 66557-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed SKOV-3 cells using HE4 antibody (66557-1-Ig, Clone: 5B2D9 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

HE4 Monoclonal antibody

HE4 Monoclonal Antibody for IHC, IF/ICC, ELISA
Cat No. 66557-1-Ig

产品说明书

CloneNo. 5B2D9

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IHC, IF/ICC, ELISA

别名

WFDC2, 5B2D9, Epididymal secretory protein E4, Major epididymis-specific protein E4, WAP four disulfide core domain protein 2

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inhuman ovary tumor tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inSKOV-3 cells
Planning an IHC experiment? We recommend our IHCeasy HE4 Ready-To-Use IHC Kit. HE4 primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:1000-1:5000
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66557-1-Ig targets HE4 in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, IF/ICC, ELISA Application Description
经测试反应性 human
免疫原

CatNo: Ag5936

Product name: Recombinant human HE4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 21-124 aa of BC046106

Sequence: GFTLVSGTGAEKTGVCPELQADQNCTQECVSDSECADNLKCCSAGCATFCSLPNDKEGSCPQVNINFPQLGLCRDQCQVDSQCPGQMKCCRNGCGKVSCVTPNF

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 WAP four-disulfide core domain 2
别名 WFDC2, 5B2D9, Epididymal secretory protein E4, Major epididymis-specific protein E4, WAP four disulfide core domain protein 2
计算分子量 13 kDa
GenBank蛋白编号BC046106
基因名称 HE4
Gene ID (NCBI) 10406
RRIDAB_2881918
偶联类型 Unconjugated
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ14508
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

WFDC2, also named as HE4, is a member of the WFDC domain family. The WFDC domain, or WAP Signature motif, contains eight cysteines forming four disulfide bonds at the core of the protein, and functions as a protease inhibitor in many family members. HE4 is expressed in a number of normal tissues, and it is also highly expressed in a number of tumors cells lines, such ovarian, colon, breast, lung and renal cells lines. In some study, HE4 was referred to as a biomarker for ovarian carcinoma.

实验方案

Product Specific Protocols
IF protocol for HE4 antibody 66557-1-IgDownload protocol
IHC protocol for HE4 antibody 66557-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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