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验证数据展示

  • WB analysis of human heart using 16216-1-AP (same clone as 16216-1-PBS)

    WB analysis of human heart using 16216-1-AP (same clone as 16216-1-PBS)

    human heart tissue were subjected to SDS PAGE followed by western blot with 16216-1-AP (HBB antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

  • IHC staining of human liver using 16216-1-AP (same clone as 16216-1-PBS)

    IHC staining of human liver using 16216-1-AP (same clone as 16216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16216-1-AP (HBB antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HBB antibody included
  • IHC staining of human liver using 16216-1-AP (same clone as 16216-1-PBS)

    IHC staining of human liver using 16216-1-AP (same clone as 16216-1-PBS)

    Immunohistochemical analysis of paraffin-embedded human liver using 16216-1-AP (HBB antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with HBB antibody included
  • IF Staining of human appendicitis using 16216-1-AP (same clone as 16216-1-PBS)

    IF Staining of human appendicitis using 16216-1-AP (same clone as 16216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed human appendicitis tissue using HBB antibody (16216-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

  • IF Staining of human placenta using 16216-1-AP (same clone as 16216-1-PBS)

    IF Staining of human placenta using 16216-1-AP (same clone as 16216-1-PBS)

    Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded human placenta tissue using HBB antibody (16216-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

  • FC experiment of K-562 using 16216-1-AP (same clone as 16216-1-PBS)

    FC experiment of K-562 using 16216-1-AP (same clone as 16216-1-PBS)

    1X10^6 K-562 cells were intracellularly stained with 0.8 ug Anti-Human HBB (16216-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.8 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP, Clone: ) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer. This data was developed using the same antibody clone with 16216-1-PBS in a different storage buffer formulation.

HBB Polyclonal antibody, PBS Only

HBB Polyclonal Antibody for WB, IHC, IF-P, FC (Intra), Indirect ELISA
Cat No. 16216-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, IHC, IF-P, FC (Intra), Indirect ELISA

别名

Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy HBB Ready-To-Use IHC Kit. HBB primary antibody included.

产品信息

16216-1-PBS targets HBB in WB, IHC, IF-P, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, IHC, IF-P, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag9216

Product name: Recombinant human HBB protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-147 aa of BC007075

Sequence: MVHLTPEEKSAVTALWGKVNVDEVGGEALGRLLVVYPWTQRFFESFGDLSTPDAVMGNPKVKAHGKKVLGAFSDGLAHLDNLKGTFATLSELHCDKLHVDPENFRLLGNVLVCVLAHHFGKEFTPPVQAAYQKVVAGVANALAHKYH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 hemoglobin, beta
别名 Spinorphin, LVV-hemorphin-7, Hemoglobin subunit beta, Hemoglobin beta chain, Beta-globin
计算分子量 147 aa, 16 kDa
观测分子量 13-16 kDa
GenBank蛋白编号BC007075
基因名称 HBB
Gene ID (NCBI) 3043
RRIDAB_10598329
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP68871
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

The hemoglobin molecule is a tetramer consisting of two alpha- and two beta-globin-like chains. HBB gene encodes hemoglobin beta chain. The hemoglobin chains, each with its own heme moiety, cooperate in binding and release of oxygen. Hemoglobin monomers have been found in blood and other tissues including macrophages, alveolar epithelial cells and brain. Defects in HBB have been linked to diseases including Sickle cell anemia, Beta-thalassemia, and Heinz body anemias. This antibody, raised against the full-length human HBB, recognizes the 13-16 kDa HBB in western blot analyses of human heart and placenta.

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