• All
  • Primary Antibodies
  • Conjugated Antibodies for IF
  • Conjugated Antibodies for FC
  • Secondary Antibodies
  • Antibody Labeling KitsNew
  • ELISA Kits
  • IHC Kits
  • Magnetic Cell Separation Kits
  • Cytokines & Growth Factors
  • Neutralizing/activating Antibodies
  • Nanobody-based Reagents
  • Accessory Products and Kits
  • Fusion Proteins
×
×
Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • IF Staining of Transfected HEK-293 using CL647-51064-2

    IF Staining of Transfected HEK-293 using CL647-51064-2

    Immunofluorescent analysis of (-20°C Ethanol) fixed Transfected HEK-293 cells using CoraLite® Plus 647 HA tag antibody (CL647-51064-2) at dilution of 1:200.

CoraLite® Plus 647-conjugated HA tag Polyclonal antibody

HA tag Polyclonal Antibody for IF/ICC, ELISA
Cat No. CL647-51064-2

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

recombinant protein

应用

IF/ICC, ELISA

别名

HA, YPYDVPDYA

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格价格库存

点击咨询售前客服

点击咨询售后客服

经过测试的应用

Positive IF/ICC detected inTransfected HEK-293 cells
For other applications, we recommend the unconjugated version of this antibody, 51064-2-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-51064-2 targets HA tag in IF/ICC, ELISA applications and shows reactivity with recombinant protein samples.

经测试应用 IF/ICC, ELISA Application Description
经测试反应性 recombinant protein
免疫原 Peptide 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 HA tag
别名 HA, YPYDVPDYA
计算分子量 1 kDa
基因名称
Gene ID (NCBI)
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 HA tag antibody CL647-51064-2Download protocol
Standard Protocols
Click here to view our Standard Protocols