HA tag Polyclonal antibody

HA tag Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

recombinant protein and More (2)

Applications

IF, IP, WB, ELISA and More (3)

Conjugate

Unconjugated

Cat No : 51064-2-AP

Print datasheet

Synonyms

HA tag, YPYDVPDYA



经过测试的应用

Positive WB detected inrecombinant protein, Transfected HEK-293 cells
Positive IP detected inTransfected HEK-293 cells
Positive IF detected inTransfected HEK-293 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for IP and 1:1000-1:4000 for WB
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

产品信息

51064-2-AP targets HA tag in WB, IP, IHC, IF, CoIP, ChIP, ELISA applications and shows reactivity with recombinant protein samples.

Tested Applications IF, IP, WB, ELISA
Cited Applications ChIP, CoIP, IF, IHC, IP, WB
Tested Reactivity recombinant protein
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Peptide 种属同源性预测
Full Name HA tag
Synonyms HA tag, YPYDVPDYA
Calculated molecular weight 1 kDa
Gene symbol
Gene ID (NCBI)
RRIDAB_11042321
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Aliquoting is unnecessary for -20oC storage.

背景介绍

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.

实验方案

Product Specific Protocols
WB protocol for HA tag antibody 51064-2-APDownload protocol
IF protocol for HA tag antibody 51064-2-APDownload protocol
IP protocol for HA tag antibody 51064-2-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
WB

Cell

RetroCHMP3 blocks budding of enveloped viruses without blocking cytokinesis.

Authors - Lara Rheinemann
WB

Cell

Glycogen accumulation and phase separation drives liver tumor initiation.

Authors - Qingxu Liu
WB

Cell

The Human RNA-Binding Proteome and Its Dynamics during Translational Arrest.

Authors - Jakob Trendel
WB

Nat Genet

Pathogenic SPTBN1 variants cause an autosomal dominant neurodevelopmental syndrome.

Authors - Margot A Cousin
IF

Immunity

Intestinal Tuft-2 cells exert antimicrobial immunity via sensing bacterial metabolite N-undecanoylglycine.

Authors - Zhen Xiong
WB

Nat Cell Biol

AIDA directly connects sympathetic innervation to adaptive thermogenesis by UCP1.

Authors - Meng Shi