验证数据展示
经过测试的应用
| Positive FC detected in | Human peripheral blood erythrocytes |
Planning an IHC experiment? We recommend our IHCeasy Glycophorin A Ready-To-Use IHC Kit. Glycophorin A primary antibody included.
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Flow Cytometry (FC) | FC : 0.25 ug per 10^6 cells in a 100 µl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
Biotin-98370-2 targets Glycophorin A/CD235a in FC applications and shows reactivity with human samples.
| 经测试应用 | FC Application Description |
| 经测试反应性 | human |
| 免疫原 | Glycophorin A/CD235a fusion protein Eg2336 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | glycophorin A (MNS blood group) |
| 别名 | Glycophorin A, CD235a, Glycophorin-A, GPA, GPErik |
| 计算分子量 | 150 aa, 16 kDa |
| GenBank蛋白编号 | BC005319 |
| 基因名称 | Glycophorin A |
| Gene ID (NCBI) | 2993 |
| 偶联类型 | Biotin |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | P02724 |
| 储存缓冲液 | PBS with 0.09% sodium azide and 0.5% BSA, pH 7.3. |
| 储存条件 | Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment. |
背景介绍
Glycophorin A (GYPA) is the major transmembrane sialoglycoprotein in erythrocytes. It is a dimeric type I transmembrane protein carrying 15 closely clustered O-linked tetrasaccharides capped with sialic acid/N-acetylneuraminic acid (Neu5Ac). This 36 kDa protein represents the major sialoglycoprotein of the red blood cell membrane displaying about one million copies per cell. (PMID: 9490702)
实验方案
| Product Specific Protocols | |
|---|---|
| FC protocol for Biotin Glycophorin A/CD235a antibody Biotin-98370-2 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |