CoraLite® Plus 488-conjugated Glucagon Monoclonal antibody
Glucagon Monoclonal Antibody for IF-P
Host / Isotype
Mouse / IgG1
Reactivity
Human, mouse
Applications
IF-P
Conjugate
CoraLite® Plus 488 Fluorescent Dye
CloneNo.
2A8G1
验证数据展示
经过测试的应用
| Positive IF detected in | mouse pancreas tissue |
Planning an IHC experiment? We recommend our IHCeasy Glucagon Ready-To-Use IHC Kit. Glucagon primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 67286-1-Ig
推荐稀释比
| Application | Dilution |
|---|---|
| Immunofluorescence (IF) | IF : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
发表文章中的应用
| IF | See 1 publications below |
产品信息
CL488-67286 targets Glucagon in IF applications and shows reactivity with Human, mouse samples.
| Tested Applications | IF-P |
| Cited Applications | IF |
| Tested Reactivity | Human, mouse |
| Cited Reactivity | mouse |
| Immunogen | Glucagon fusion protein Ag10629 种属同源性预测 |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Full Name | glucagon |
| Synonyms | GCG, GLP 2, GLP1, GLP2, glucagon, GRPP, GRPP Oxyntomodulin, OXM |
| Calculated Molecular Weight | 180 aa, 21 kDa |
| GenBank Accession Number | BC005278 |
| Gene Symbol | GCG |
| Gene ID (NCBI) | 2641 |
| RRID | AB_2883402 |
| Conjugate | CoraLite® Plus 488 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 493 nm / 522 nm |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P01275 |
| Storage Buffer | PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20oC storage. |
背景介绍
Glucagon is a 29-amino acid peptide hormone secreted from the pancreatic alpha cells with a powerful stimulatory effect on hepatic glucose production acting to increase plasma glucose levels. Glucagon is best known as the counter-regulatory hormone to INS, and normal glucose homeostasis depends largely on the balanced secretion of INS and glucagon fromthe pancreatic beta and alpha cells, respectively.The regulation of glucose metabolism by glucagon is mediated by its direct action on the peripheral tissues such as the liver and also by the brain. Glucagon is also released postprandially in a transient manner, which was shown to be involved in inhibition of food intake via reduction of meal size.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL Plus 488 Glucagon antibody CL488-67286 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
iScience Optimal HSF1 activation in response to acute cold stress in BAT requires nuclear TXNIP |