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验证数据展示

  • FC experiment of HepG2 using CL647-10566

    FC experiment of HepG2 using CL647-10566

    1X10^6 HepG2 cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GYS1 (CL647-10566) (red), or 0.2 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • IF Staining of HepG2 using CL647-10566

    IF Staining of HepG2 using CL647-10566

    Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 647 GYS1 antibody (CL647-10566) at dilution of 1:200.

  • Featured Product
  • KD/KO验证

CoraLite® Plus 647-conjugated GYS1 Polyclonal antibody

GYS1 Polyclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL647-10566

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse, rat

应用

IF/ICC, FC (Intra)

别名

GS, Glycogen synthase1, glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  CoraLite® Plus 647
规格: 

-/ -

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经过测试的应用

Positive IF/ICC detected inHepG2 cells
Positive FC (Intra) detected inHepG2 cells
Planning an IHC experiment? We recommend our IHCeasy GYS1 Ready-To-Use IHC Kit. GYS1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 10566-1-AP

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-10566 targets GYS1 in IF/ICC, FC (Intra) applications and shows reactivity with human, mouse, rat samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human, mouse, rat
免疫原 GYS1 fusion protein Ag0857 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 glycogen synthase 1 (muscle)
别名 GS, Glycogen synthase1, glycogen synthase 1 (muscle), Glycogen synthase 1, Glycogen
计算分子量 84 kDa
观测分子量 84 kDa
GenBank蛋白编号BC007688
基因名称 GYS1
Gene ID (NCBI) 2997
RRIDAB_2934825
偶联类型 CoraLite® Plus 647 Fluorescent Dye
最大激发/发射波长654 nm / 674 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDP13807
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA , pH 7.3
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GYS1(Glycogen [starch] synthase, muscle) is the the rate limiting enzyme of the insulin-induced glycogenesis, transferring glucose units from UDP-Glc to a glycogen primer. It catalyzes the linear addition of glucose residues to the branching structure of glycogen, providing a convenient store of glucose for times of metabolic need. This protein has 2 isoforms produced by alternative splicing.

实验方案

Product Specific Protocols
IF protocol for CL Plus 647 GYS1 antibody CL647-10566Download protocol
FC protocol for CL Plus 647 GYS1 antibody CL647-10566Download protocol
Standard Protocols
Click here to view our Standard Protocols