验证数据展示
经过测试的应用
| Positive WB detected in | HeLa cells, K-562 cells, Jurkat cells, mouse skeletal muscle tissue, rat heart tissue, rat skeletal muscle tissue |
| Positive IHC detected in | human liver tissue, human skeletal muscle tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
10566-1-AP targets GYS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| 经测试应用 | WB, IHC, IF/ICC, ELISA Application Description |
| 文献引用应用 | WB, IHC, IF |
| 经测试反应性 | human, mouse, rat |
| 文献引用反应性 | human, mouse, rat |
| 免疫原 |
CatNo: Ag0857 Product name: Recombinant human GYS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 432-737 aa of BC007688 Sequence: IFATQRQSFPPVCTHNMLDDSSDPILTTIRRIGLFNSSADRVKVIFHPEFLSSTSPLLPVDYEEFVRGCHLGVFPSYYEPWGYTPAECTVMGIPSISTNLSGFGCFMEEHIADPSAYGIYILDRRFRSLDDSCSQLTSFLYSFCQQSRRQRIIQRNRTERLSDLLDWKYLGRYYMSARHMALSKAFPEHFTYEPNEADAAQGYRYPRPASVPPSPSLSRHSSPHQSEDEEDPRNGPLEEDGERYDEDEEAAKDRRNIRAPEWPRRASCTSSTSGSKRNSVDTATSSSLSTPSEPLSPTSSLGEERN 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Polyclonal |
| 产品类型 | Antibody |
| 全称 | glycogen synthase 1 (muscle) |
| 别名 | EC:2.4.1.11, Glycogen, Glycogen synthase 1, glycogen synthase 1 (muscle), Glycogen synthase1 |
| 计算分子量 | 84 kDa |
| 观测分子量 | 84 kDa |
| GenBank蛋白编号 | BC007688 |
| 基因名称 | GYS1 |
| Gene ID (NCBI) | 2997 |
| RRID | AB_2116401 |
| 偶联类型 | Unconjugated |
| 形式 | Liquid |
| 纯化方式 | Antigen affinity purification |
| UNIPROT ID | P13807 |
| 储存缓冲液 | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| 储存条件 | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GYS1(Glycogen [starch] synthase, muscle) is the the rate limiting enzyme of the insulin-induced glycogenesis, transferring glucose units from UDP-Glc to a glycogen primer. It catalyzes the linear addition of glucose residues to the branching structure of glycogen, providing a convenient store of glucose for times of metabolic need. This protein has 2 isoforms produced by alternative splicing.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for GYS1 antibody 10566-1-AP | Download protocol |
| IHC protocol for GYS1 antibody 10566-1-AP | Download protocol |
| WB protocol for GYS1 antibody 10566-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
发表文章
| Species | Application | Title |
|---|---|---|
Nat Commun Multi-layered transcriptional control of glycogen metabolism coordinates thermogenic remodeling of white adipocytes in male mice. | ||
Metabolism Recurrent hypoglycemia increases hepatic gluconeogenesis without affecting glycogen metabolism or systemic lipolysis in rat | ||
Mol Cell Glucose-1-phosphate promotes compartmentalization of glycogen with the pentose phosphate pathway in CD8+ memory T cells | ||
Int J Biol Sci PYGL-mediated glucose metabolism reprogramming promotes EMT phenotype and metastasis of pancreatic cancer | ||
Phytomedicine Radix Astragali decoction improves liver regeneration by upregulating hepatic expression of aquaporin-9 | ||
Phytomedicine Astragaloside IV and cycloastragenol promote liver regeneration through regulation of hepatic oxidative homeostasis and glucose/lipid metabolism |
