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验证数据展示

  • WB analysis using 80918-1-RR

    WB analysis using 80918-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80918-1-RR (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of RAW 264.7 using 80918-1-RR

    FC experiment of RAW 264.7 using 80918-1-RR

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human GSDMD (80918-1-RR, Clone:2N24) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

  • Affinity and Kinetic Characterization of 80918-1-RR

    Affinity and Kinetic Characterization of 80918-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80918-1-RR against Human GSDMD were performed. The affinity constant is below 1 pM.

GSDMD Recombinant antibody

GSDMD Recombinant Antibody for WB, FC (Intra), ELISA
Cat No. 80918-1-RR

产品说明书

CloneNo. 2N24

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), ELISA

别名

2N24, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
Unconjugated
CoraLite® Plus 488
规格价格库存

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经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells
Positive FC (Intra) detected inRAW 264.7 cells
Planning an IHC experiment? We recommend our IHCeasy GSDMD Ready-To-Use IHC Kit. GSDMD primary antibody included.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:1000-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80918-1-RR targets GSDMD in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), ELISA Application Description
经测试反应性 human, mouse
免疫原 GSDMD fusion protein Ag14757 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 gasdermin D
别名 2N24, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD
计算分子量 484 aa, 53 kDa
观测分子量50-53 kDa
GenBank蛋白编号BC008904
基因名称 GSDMD
Gene ID (NCBI) 79792
RRIDAB_2918919
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57764
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

实验方案

Product Specific Protocols
WB protocol for GSDMD antibody 80918-1-RRDownload protocol
FC protocol for GSDMD antibody 80918-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols