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GSDMD Recombinant antibody

GSDMD Recombinant Antibody for FC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, mouse

Applications

FC, WB, ELISA

Conjugate

Unconjugated

CloneNo.

2N24

Cat No : 80918-1-RR

Print datasheet

Synonyms

DF5L, DFNA5L, FKSG10, FLJ12150, gasdermin D, GSDMD, GSDMDC1

验证数据展示

  • WB analysis using 80918-1-RR

    WB analysis using 80918-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 80918-1-RR (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • FC experiment of RAW 264.7 using 80918-1-RR

    FC experiment of RAW 264.7 using 80918-1-RR

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human GSDMD (80918-1-RR, Clone:2N24) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive WB detected inHeLa cells, A549 cells, Jurkat cells
Positive FC detected inRAW 264.7 cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

80918-1-RR targets GSDMD in WB, FC, ELISA applications and shows reactivity with Human, mouse samples.

Tested Applications FC, WB, ELISA
Tested Reactivity Human, mouse
Immunogen GSDMD fusion protein Ag14757 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name gasdermin D
Synonyms DF5L, DFNA5L, FKSG10, FLJ12150, gasdermin D, GSDMD, GSDMDC1
Calculated Molecular Weight 484 aa, 53 kDa
Observed Molecular Weight50-53 kDa
GenBank Accession NumberBC008904
Gene Symbol GSDMD
Gene ID (NCBI) 79792
RRIDAB_2918919
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDP57764
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a member of the gasdermin family. Members of this family appear to play a role in regulation of epithelial proliferation. GSDMD has been suggested to act as a tumor suppressor. Recently, GSDMD is identified as a new component of inflammasomes, and it is an executor of pyroptosis and required for interleukin-1β secretion. Cleavage of GSDMD by inflammatory caspases determines pyroptotic cell death.

实验方案

Product Specific Protocols
WB protocol for GSDMD antibody 80918-1-RRDownload protocol
FC protocol for GSDMD antibody 80918-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols