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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80918-1-RR (same clone as 80918-1-PBS)

    WB analysis using 80918-1-RR (same clone as 80918-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80918-1-RR (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80918-1-PBS in a different storage buffer formulation.

  • FC experiment of RAW 264.7 using 80918-1-RR (same clone as 80918-1-PBS)

    FC experiment of RAW 264.7 using 80918-1-RR (same clone as 80918-1-PBS)

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human GSDMD (80918-1-RR, Clone:2N24) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80918-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80918-1-RR

    Affinity and Kinetic Characterization of 80918-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80918-1-RR against Human GSDMD were performed. The affinity constant is below 1 pM.

GSDMD Recombinant antibody, PBS Only

GSDMD Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 80918-1-PBS

产品说明书

CloneNo. 2N24

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

2N24, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSDMD Ready-To-Use IHC Kit. GSDMD primary antibody included.

产品信息

80918-1-PBS targets GSDMD in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原 GSDMD fusion protein Ag14757 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 gasdermin D
别名 2N24, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD
计算分子量 484 aa, 53 kDa
观测分子量50-53 kDa
GenBank蛋白编号BC008904
基因名称 GSDMD
Gene ID (NCBI) 79792
RRIDAB_2918919
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57764
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.