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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 80918-1-RR (same clone as 80918-1-PBS)

    WB analysis using 80918-1-RR (same clone as 80918-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 80918-1-RR (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 80918-1-PBS in a different storage buffer formulation.

  • FC experiment of RAW 264.7 using 80918-1-RR (same clone as 80918-1-PBS)

    FC experiment of RAW 264.7 using 80918-1-RR (same clone as 80918-1-PBS)

    1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human GSDMD (80918-1-RR, Clone:2N24) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 80918-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 80918-1-RR

    Affinity and Kinetic Characterization of 80918-1-RR

    Biolayer interferometry (BLl) kinetic assays of 80918-1-RR against Human GSDMD were performed. The affinity constant is below 1 pM.

    {{ptg:ProductFeaturesInfo}}

GSDMD Recombinant monoclonal antibody, PBS Only

GSDMD Uni-rAb® Recombinant Antibody for WB, FC (Intra), Indirect ELISA
Cat No. 80918-1-PBS

产品说明书

CloneNo. 2N24

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

WB, FC (Intra), Indirect ELISA

别名

2N24, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSDMD Ready-To-Use IHC Kit. GSDMD primary antibody included.

产品信息

80918-1-PBS targets GSDMD in WB, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 WB, FC (Intra), Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag14757

Product name: Recombinant human GSDMD protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 135-484 aa of BC008904

Sequence: HERHLRQPEHKVLQQLRSRGDNVYVVTEVLQTQKEVEVTRTHKREGSGRFSLPGATCLQGEGQGHLSQKKTVTIPSGSTLAFRVAQLVIDSDLDVLLFPDKKQRTFQPPATGHKRSTSEGAWPQLPSGLSMMRCLHNFLTDGVPAEGAFTEDFQGLRAEVETISKELELLDRELCQLLLEGLEGVLRDQLALRALEEALEQGQSLGPVEPLDGPAGAVLECLVLSSGMLVPELAIPVVYLLGALTMLSETQHKLLAEALESQTLLGPLELVGSLLEQSAPWQERSTMSLPPGLLGNSWGEGAPAWVLLDECGLELGEDTPHVCWEPQAQGRMCALYASLALLSGLSQEPH

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 gasdermin D
别名 2N24, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD
计算分子量 484 aa, 53 kDa
观测分子量50-53 kDa
GenBank蛋白编号BC008904
基因名称 GSDMD
Gene ID (NCBI) 79792
RRIDAB_2918919
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57764
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.