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Host
0
Species Reactivity
0
Species Reactivity
0
Conjugate
0
Conjugate
0
Compatible Species
0
Conjugate
0

验证数据展示

  • WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

    WB analysis using 66387-1-Ig (same clone as 66387-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 66387-1-Ig (GSDMD antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

  • IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)

    IF Staining of HeLa using 66387-1-Ig (same clone as 66387-1-PBS)

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using GSDMD antibody (66387-1-Ig, Clone: 2E6C7 ) at dilution of 1:800 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

  • IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)

    IP experiment of THP-1 using 66387-1-Ig (same clone as 66387-1-PBS)

    IP result of anti-GSDMD (IP:66387-1-Ig, 4ug; Detection:66387-1-Ig 1:2000) with THP-1 cells lysate 1560 ug. This data was developed using the same antibody clone with 66387-1-PBS in a different storage buffer formulation.

GSDMD Monoclonal antibody, PBS Only

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 66387-1-PBS

产品说明书

CloneNo. 2E6C7

宿主/亚型

Mouse / IgG2a

种属反应性

human

应用

WB, IF/ICC, IP, ELISA

别名

2E6C7, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存

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Planning an IHC experiment? We recommend our IHCeasy GSDMD Ready-To-Use IHC Kit. GSDMD primary antibody included.

产品信息

66387-1-PBS targets GSDMD in WB, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
经测试反应性 human
免疫原 GSDMD fusion protein Ag16827 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 gasdermin D
别名 2E6C7, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD
计算分子量 484 aa, 53 kDa
观测分子量 47-53 kDa
GenBank蛋白编号BC008904
基因名称 GSDMD
Gene ID (NCBI) 79792
RRIDAB_2881763
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57764
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.