GSDMD Monoclonal antibody

GSDMD Monoclonal Antibody for WB, IF/ICC, IP, ELISA
Cat No. 66387-1-Ig

产品说明书

CloneNo. 2E6C7

宿主/亚型

Mouse / IgG2a

种属反应性

human and More (2)

应用

WB, IF/ICC, IP, ELISA and More (2)

2E6C7, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD

缓冲液配方:  PBS and Azide
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格价格库存


经过测试的应用

Positive WB detected inHEK-293 cells, A431 cells, COLO 320 cells, EC109 cells, HL-60 cells, human colon tissue, human small intestine tissue, K-562 cells, Raji cells, TF-1 cells, PC-3 cells, U2OS cells, A549 cells, HepG2 cells, Jurkat cells
Positive IP detected inTHP-1 cells
Positive IF/ICC detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GSDMD Ready-To-Use IHC Kit. GSDMD primary antibody included.
Planning an IF experiment? We recommend our CoraLite® Plus 488 and CoraLite®594 conjugated versions of this antibody.

推荐稀释比

应用推荐稀释比
Western Blot (WB)WB : 1:5000-1:50000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunofluorescence (IF)/ICCIF/ICC : 1:400-1:1600
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

66387-1-Ig targets GSDMD in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

经测试应用 WB, IF/ICC, IP, ELISA Application Description
文献引用应用WB, IHC, IF
经测试反应性 human
文献引用反应性human, mouse, rat
免疫原 GSDMD fusion protein Ag16827 种属同源性预测
宿主/亚型 Mouse / IgG2a
抗体类别 Monoclonal
产品类型 Antibody
全称 gasdermin D
别名 2E6C7, FKSG10, gasdermin D, Gasdermin domain-containing protein 1, gasderminD
计算分子量 484 aa, 53 kDa
观测分子量 47-53 kDa
GenBank蛋白编号BC008904
基因名称 GSDMD
Gene ID (NCBI) 79792
RRIDAB_2881763
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDP57764
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GSDMD is a 53 kDa cytosolic protein and a member of the gasdermin protein family, which features 6 members in humans (GSDMA, -B, -C, -D, -E and -F. GSDMD was identified as the executioner of inflammasome-associated pyroptosis. Caspase-1, -11, or -4 cleave GSDMD after an aspartate residue within a central linker domain (D275 in humans and D276 in mice) to generate a 31-kDa N-terminal GSDMD fragment (GSDMD-NT) and a 22-kDa C-terminal fragment (GSDMD-CT). In addition to the canonical 31/22 kDa cleavage products, a 43 kDa fragment has been observed under specific experimental or pathological conditions. The generation of the 43 kDa fragment was observed upon caspase-3 and -7 activation during apoptosis. This antibody can only detect full-length GSDMD.

实验方案

Product Specific Protocols
WB protocol for GSDMD antibody 66387-1-IgDownload protocol
IF protocol for GSDMD antibody 66387-1-IgDownload protocol
IP protocol for GSDMD antibody 66387-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
mouseWB

Mol Cancer

Metal-enriched HSP90 nanoinhibitor overcomes heat resistance in hyperthermic intraperitoneal chemotherapy used for peritoneal metastases

Authors - Qiang Wang
humanWB

PLoS Pathog

An NLRP3 inflammasome-triggered cytokine storm contributes to Streptococcal toxic shock-like syndrome (STSLS).

Authors - Lan Lin
humanWB

iScience

ASC/inflammasome-independent pyroptosis in ovarian cancer cells through translational augmentation of caspase-1

Authors - Ozlem Calbay
mouseWB

Biochem Pharmacol

Deficiency of purinergic P2X4 receptor alleviates experimental autoimmune hepatitis in mice

Authors - Zejin Liu
humanWB

Emerg Microbes Infect

Acquiring high expression of suilysin enable non-epidemic Streptococccus suis to cause Streptococcal toxic shock-like syndrome (STSLS) through NLRP3 inflammasome hyperactivation.

Authors - Lei Xu
mouseWB

Mol Med

MALAT1 knockdown alleviates the pyroptosis of microglias in diabetic cerebral ischemia via regulating STAT1 mediated NLRP3 transcription

Authors - Nan Zhao
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