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CoraLite® Plus 647-conjugated GS28 Polyclonal antibody

GS28 Polyclonal Antibody for FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Conjugate

CoraLite® Plus 647 Fluorescent Dye

Cat No : CL647-16106

Print datasheet

Synonyms

28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28

验证数据展示

  • FC experiment of HeLa using CL647-16106

    FC experiment of HeLa using CL647-16106

    1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human GS28 (CL647-16106) (red), or 0.2 ug isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

经过测试的应用

Positive FC detected inHeLa cells
For other applications, we recommend the unconjugated version of this antibody, 16106-1-AP

推荐稀释比

ApplicationDilution
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL647-16106 targets GS28 in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

Tested Applications FC (Intra)
Tested Reactivity human, mouse, rat
Immunogen GS28 fusion protein Ag9021 种属同源性预测
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Full Name golgi SNAP receptor complex member 1
Synonyms 28 kDa cis Golgi SNARE p28, 28 kDa Golgi SNARE protein, GOLIM2, GOS 28, GOS28, GOS28/P28, GOSR1, GS28, P28
Calculated Molecular Weight28.6 kDa
Observed Molecular Weight 28 kDa
GenBank Accession NumberBC012620
Gene Symbol GOSR1
Gene ID (NCBI) 9527
Conjugate CoraLite® Plus 647 Fluorescent Dye
Excitation/Emission Maxima Wavelengths654 nm / 674 nm
Form Liquid
Purification MethodAntigen affinity purification
UNIPROT IDO95249
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GS28, also known as GOSR1, is a 28-kDa Golgi SNARE protein that participates in ER-Golgi and intra-Golgi transport. GS28 is considered to be a core component of the Golgi SNARE complex. (PMID: 8638159)

实验方案

Product Specific Protocols
FC protocol for CL Plus 647 GS28 antibody CL647-16106Download protocol
Standard Protocols
Click here to view our Standard Protocols