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GRB10 Recombinant antibody

GRB10 Recombinant Antibody for IF, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human, Mouse, Rat

Applications

IF, WB, ELISA

Conjugate

Unconjugated

CloneNo.

1D13

Cat No : 81225-1-RR

Print datasheet

Synonyms

Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, RSS

验证数据展示

  • WB analysis using 81225-1-RR

    WB analysis using 81225-1-RR

    Various lysates were subjected to SDS PAGE followed by western blot with 81225-1-RR (GRB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • IF Staining of LNCaP using 81225-1-RR

    IF Staining of LNCaP using 81225-1-RR

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using GRB10 antibody (81225-1-RR, Clone: 1D13 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

经过测试的应用

Positive WB detected inPC-3 cells, NIH/3T3 cells, HSC-T6 cells
Positive IF detected inLNCaP cells

推荐稀释比

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

81225-1-RR targets GRB10 in WB, IF, ELISA applications and shows reactivity with Human, Mouse, Rat samples.

Tested Applications IF, WB, ELISA
Tested Reactivity Human, Mouse, Rat
Immunogen GRB10 fusion protein Ag20355 种属同源性预测
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Full Name growth factor receptor-bound protein 10
Synonyms Grb 10, GRB IR, GRB10, GRB10 adapter protein, GRBIR, IRBP, KIAA0207, MEG1, RSS
Calculated Molecular Weight536aa,61 kDa; 594aa,67 kDa
Observed Molecular Weight60-70 kDa
GenBank Accession NumberBC024285
Gene Symbol GRB10
Gene ID (NCBI) 2887
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
UNIPROT IDQ13322
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation. GRB10 has 4 isoforms with the molecular mass of 60-70 kDa, which are produced by alternative splicing.

实验方案

Product Specific Protocols
WB protocol for GRB10 antibody 81225-1-RRDownload protocol
IF protocol for GRB10 antibody 81225-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols