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验证数据展示

  • WB analysis using 81225-1-RR (same clone as 81225-1-PBS)

    WB analysis using 81225-1-RR (same clone as 81225-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 81225-1-RR (GRB10 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 81225-1-PBS in a different storage buffer formulation.

  • IF Staining of LNCaP using 81225-1-RR (same clone as 81225-1-PBS)

    IF Staining of LNCaP using 81225-1-RR (same clone as 81225-1-PBS)

    Immunofluorescent analysis of (-20°C Methanol) fixed LNCaP cells using GRB10 antibody (81225-1-RR, Clone: 1D13 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 81225-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 81225-1-RR

    Affinity and Kinetic Characterization of 81225-1-RR

    Biolayer interferometry (BLl) kinetic assays of 81225-1-RR against Human GRB10 were performed. The affinity constant is 0.195 nM.

GRB10 Recombinant monoclonal antibody, PBS Only

GRB10 Uni-rAb® Recombinant Antibody for WB, IF/ICC, Indirect ELISA
Cat No. 81225-1-PBS

产品说明书

CloneNo. 1D13

宿主/亚型

Rabbit / IgG

种属反应性

rat, mouse, human

应用

WB, IF/ICC, Indirect ELISA

别名

KIAA0207, Insulin receptor-binding protein Grb-IR, Growth factor receptor-bound protein 10, GRBIR, 1D13

缓冲液配方:  PBS Only
PBS, Azide, Glycerol
PBS Only
偶联物:  Unconjugated
规格价格库存

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产品信息

81225-1-PBS targets GRB10 in WB, IF/ICC, Indirect ELISA applications and shows reactivity with rat, mouse, human samples.

经测试应用 WB, IF/ICC, Indirect ELISA Application Description
经测试反应性 rat, mouse, human
免疫原

CatNo: Ag20355

Product name: Recombinant human GRB10 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 1-80 aa of BC024285

Sequence: MNASLESLYSACSMQSDTVPLLQNGQHARSQPRASGPPRSIQPQVSPRQRVQRSQPVHILAVRRLQEEDQQFRTSSLPAI

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 growth factor receptor-bound protein 10
别名 KIAA0207, Insulin receptor-binding protein Grb-IR, Growth factor receptor-bound protein 10, GRBIR, 1D13
计算分子量536aa,61 kDa; 594aa,67 kDa
观测分子量60-70 kDa
GenBank蛋白编号BC024285
基因名称 GRB10
Gene ID (NCBI) 2887
偶联类型 Unconjugated
形式Liquid
纯化方式Protein A purification
UNIPROT IDQ13322
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GRB10 is an adapter protein which modulates coupling of a number of cell surface receptor kinases with specific signaling pathways. GRB10 has three consensus domains including pleckstrin homology (PH) domain, SH2/SH3 domain and Ras-associating domain. By binding to kinases, GRB10 suppresses signals from activated receptors tyrosine kinases, including INSR and IGF1R. It may play a role in mediating ubiquitination of INSR, leading to proteasomal degradation. GRB10 has 4 isoforms with the molecular mass of 60-70 kDa, which are produced by alternative splicing.

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