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CoraLite® Plus 488-conjugated GPT/ALT1 Monoclonal antibody

GPT/ALT1 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG1

Reactivity

Human, Pig

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

5F6B5

Cat No : CL488-67531

Print datasheet

Synonyms

AAT1, Alanine aminotransferase 1, ALT1, GPT, GPT 1, GPT1

验证数据展示

  • IF Staining of HepG2 using CL488-67531

    IF Staining of HepG2 using CL488-67531

    Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite® Plus 488 GPT/ALT1 antibody (CL488-67531, Clone: 5F6B5 ) at dilution of 1:200.

经过测试的应用

Positive IF detected inHepG2 cells
For other applications, we recommend the unconjugated version of this antibody, 67531-1-Ig

推荐稀释比

ApplicationDilution
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL488-67531 targets GPT/ALT1 in IF applications and shows reactivity with Human, Pig samples.

Tested Applications IF/ICC
Tested Reactivity Human, Pig
Immunogen GPT/ALT1 fusion protein Ag10453 种属同源性预测
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Full Name glutamic-pyruvate transaminase (alanine aminotransferase)
Synonyms AAT1, Alanine aminotransferase 1, ALT1, GPT, GPT 1, GPT1
Calculated Molecular Weight 496 aa, 55 kDa
Observed Molecular Weight 52 kDa
GenBank Accession NumberBC018207
Gene Symbol GPT
Gene ID (NCBI) 2875
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
UNIPROT IDP24298
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPT, also known as ALT1 (glutamate-pyruvate transaminase 1), catalyzes the reversible transamination between alanine and 2-oxoglutarate to generate pyruvate and glutamate and, therefore, plays a key role in the intermediary metabolism of glucose and amino acids. Serum activity levels of this enzyme are routinely used as a biomarker of liver injury caused by drug toxicity, infection, alcohol, and steatosis. A related gene on chromosome 16 encodes a putative mitochondrial alanine aminotransaminase.

实验方案

Product Specific Protocols
IF protocol for CL Plus 488 GPT/ALT1 antibody CL488-67531Download protocol
Standard Protocols
Click here to view our Standard Protocols