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验证数据展示

  • FC experiment of RAW 264.7 using FITC-20306

    FC experiment of RAW 264.7 using FITC-20306

    1x10^6 RAW 264.7 cells were intracellularly stained with 0.8 ug FITC-conjugated GPR65 Polyclonal antibody (FITC-20306)(red), or 0.8 ug Isotype Control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

FITC-conjugated GPR65 Polyclonal antibody

GPR65 Polyclonal Antibody for ELISA
Cat No. FITC-20306

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

ELISA

别名

G protein coupled receptor 65, G-protein coupled receptor 65, hTDAG8, Psychosine receptor, T-cell death-associated gene 8 protein

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  FITC
规格价格库存

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经过测试的应用

Positive FC (Intra) detected inRAW 264.7 cells

推荐稀释比

应用推荐稀释比
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.8 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

FITC-20306 targets GPR65 in ELISA applications and shows reactivity with human samples.

经测试应用 ELISA Application Description
经测试反应性 human
免疫原 GPR65 fusion protein Ag14139 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 65
别名 G protein coupled receptor 65, G-protein coupled receptor 65, hTDAG8, Psychosine receptor, T-cell death-associated gene 8 protein
计算分子量 337 aa, 39 kDa
GenBank蛋白编号BC035633
基因名称 GPR65
Gene ID (NCBI) 8477
偶联类型 FITC Fluorescent Dye
最大激发/发射波长498 nm / 526 nm
形式 Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYL9
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).

实验方案

Product Specific Protocols
FC protocol for FITC GPR65 antibody FITC-20306Download protocol
Standard Protocols
Click here to view our Standard Protocols