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验证数据展示

  • IHC staining of human tonsillitis using 20306-1-AP

    IHC staining of human tonsillitis using 20306-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human tonsillitis using 20306-1-AP

    IHC staining of human tonsillitis using 20306-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 20306-1-AP (GPR65 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR65 Polyclonal antibody

GPR65 Polyclonal Antibody for IHC, ELISA
Cat No. 20306-1-AP

产品说明书

引用文献(1)

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

IHC, ELISA and More (1)

别名

G protein coupled receptor 65, GPR65, hTDAG8, Psychosine receptor, TDAG8

缓冲液配方:  PBS and Azide
PBS and Azide
偶联物:  Unconjugated
Unconjugated
FITC
规格价格库存

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经过测试的应用

Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

20306-1-AP targets GPR65 in IHC, IF, ELISA applications and shows reactivity with human samples.

经测试应用 IHC, ELISA Application Description
文献引用应用IHC, IF
经测试反应性 human
文献引用反应性human
免疫原

CatNo: Ag14139

Product name: Recombinant human GPR65 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 238-337 aa of BC035633

Sequence: CFTPFHVMLLIRCILEHAVNFEDHSNSGKRTYTMYRITVALTSLNCVADPILYCFVTETGRYDMWNILKFCTGRCNTSQRQRKRILSVSTKDTMELEVLE

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 65
别名 G protein coupled receptor 65, GPR65, hTDAG8, Psychosine receptor, TDAG8
计算分子量 337 aa, 39 kDa
GenBank蛋白编号BC035633
基因名称 GPR65
Gene ID (NCBI) 8477
RRIDAB_2878668
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDQ8IYL9
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR65 (G protein-coupled receptor 65) was defined originally as T-cell death-associated gene 8 (TDAG8), functions as a proton-sensing receptor, and is also sensitive to psychosine (PMID: 35343079). GPR65 promotes adaptation to an acidic environment to enhance cell survival and proliferation, thereby promoting tumor development (PMID: 36852075).

实验方案

Product Specific Protocols
IHC protocol for GPR65 antibody 20306-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

发表文章

SpeciesApplicationTitle
humanIHC,IF

Neurosurg Rev

GPR65 contributes to constructing immunosuppressive microenvironment in glioma

Authors - Jikang Fan
View Article