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验证数据展示

  • IHC staining of mouse lung using 28232-1-AP (same clone as 28232-1-PBS)

    IHC staining of mouse lung using 28232-1-AP (same clone as 28232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28232-1-PBS in a different storage buffer formulation.

  • IHC staining of mouse kidney using 28232-1-AP (same clone as 28232-1-PBS)

    IHC staining of mouse kidney using 28232-1-AP (same clone as 28232-1-PBS)

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 28232-1-PBS in a different storage buffer formulation.

GPR4 Polyclonal antibody, PBS Only

GPR4 Polyclonal Antibody for IHC, Indirect ELISA
Cat No. 28232-1-PBS

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, Indirect ELISA

别名

G protein coupled receptor 4, G-protein coupled receptor 4, G-protein coupled receptor 6C.l

缓冲液配方: 
PBS, Azide, Glycerol
偶联物:  Unconjugated
规格价格库存

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产品信息

28232-1-PBS targets GPR4 in IHC, Indirect ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, Indirect ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag28273

Product name: Recombinant human GPR4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 203-362 aa of BC067535

Sequence: GILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 4
别名 G protein coupled receptor 4, G-protein coupled receptor 4, G-protein coupled receptor 6C.l
计算分子量 362 aa, 41 kDa
GenBank蛋白编号BC067535
基因名称 GPR4
Gene ID (NCBI) 2828
RRIDAB_3086039
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46093
储存缓冲液 PBS only, pH 7.3.
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GPR4 (G-protein-coupled receptor 4) is a proton-sensing G-protein-coupled receptor (GPCR) that contributes to neovascularization in acidic microenvironments (PMID: 33632325). GPR4 is highly expressed in vascular endothelial cells (ECs) and blood vessel-rich tissues such as the lung, kidney, heart, and liver. Moreover, GPR4 has been shown to mediate EC inflammatory responses to acidosis and is central for leukocyte-endothelium interaction (PMID: 27940273).

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