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验证数据展示

  • IHC staining of mouse lung using 28232-1-AP

    IHC staining of mouse lung using 28232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of mouse kidney using 28232-1-AP

    IHC staining of mouse kidney using 28232-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 28232-1-AP (GPR4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

GPR4 Polyclonal antibody

GPR4 Polyclonal Antibody for IHC, ELISA
Cat No. 28232-1-AP

产品说明书

宿主/亚型

Rabbit / IgG

种属反应性

human, mouse

应用

IHC, ELISA

别名

G protein coupled receptor 4, G-protein coupled receptor 4, G-protein coupled receptor 6C.l

缓冲液配方:  PBS, Azide, Glycerol
PBS, Azide, Glycerol
偶联物:  Unconjugated
Unconjugated
规格价格库存

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经过测试的应用

Positive IHC detected inmouse lung tissue, mouse kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

推荐稀释比

应用推荐稀释比
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

28232-1-AP targets GPR4 in IHC, ELISA applications and shows reactivity with human, mouse samples.

经测试应用 IHC, ELISA Application Description
经测试反应性 human, mouse
免疫原

CatNo: Ag28273

Product name: Recombinant human GPR4 protein

Source: e coli.-derived, PET28a

Tag: 6*His

Domain: 203-362 aa of BC067535

Sequence: GILRAVRGSVSTERQEKAKIKRLALSLIAIVLVCFAPYHVLLLSRSAIYLGRPWDCGFEERVFSAYHSSLAFTSLNCVADPILYCLVNEGARSDVAKALHNLLRFLASDKPQEMANASLTLETPLTSKRNSTAKAMTGSWAATPPSQGDQVQLKMLPPAQ

种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Polyclonal
产品类型 Antibody
全称 G protein-coupled receptor 4
别名 G protein coupled receptor 4, G-protein coupled receptor 4, G-protein coupled receptor 6C.l
计算分子量 362 aa, 41 kDa
GenBank蛋白编号BC067535
基因名称 GPR4
Gene ID (NCBI) 2828
RRIDAB_3086039
偶联类型 Unconjugated
形式Liquid
纯化方式Antigen affinity purification
UNIPROT IDP46093
储存缓冲液 PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
储存条件Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

GPR4 (G-protein-coupled receptor 4) is a proton-sensing G-protein-coupled receptor (GPCR) that contributes to neovascularization in acidic microenvironments (PMID: 33632325). GPR4 is highly expressed in vascular endothelial cells (ECs) and blood vessel-rich tissues such as the lung, kidney, heart, and liver. Moreover, GPR4 has been shown to mediate EC inflammatory responses to acidosis and is central for leukocyte-endothelium interaction (PMID: 27940273).

实验方案

Product Specific Protocols
IHC protocol for GPR4 antibody 28232-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols
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