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验证数据展示

  • IF Staining of HeLa using CL594-66331

    IF Staining of HeLa using CL594-66331

    Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®594 GP73/GOLPH2 antibody (CL594-66331, Clone: 3D1F1 ) at dilution of 1:200.

  • FC experiment of HeLa using CL594-66331

    FC experiment of HeLa using CL594-66331

    1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human GP73/GOLPH2 (CL594-66331, Clone:3D1F1) (red), or 0.4 ug Mouse IgG1 Isotype Control (CL594-66360, Clone: T1F8D3F10) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer.

  • IF Staining of HepG2 using CL594-66331

    IF Staining of HepG2 using CL594-66331

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL594-66331 (GP73/GOLPH2 antibody) at dilution of 1:100.

  • Featured Product
  • KD/KO验证

CoraLite®594-conjugated GP73/GOLPH2 Monoclonal antibody

GP73/GOLPH2 Monoclonal Antibody for IF/ICC, FC (Intra)
Cat No. CL594-66331

产品说明书

CloneNo. 3D1F1

宿主/亚型

Mouse / IgG1

种属反应性

human

应用

IF/ICC, FC (Intra)

别名

GOLM1, GOLPH2, GP73, 3D1F1, C9orf155

缓冲液配方:  PBS, Proclin 300, BSA, Glycerol
PBS, Proclin 300, BSA, Glycerol
偶联物:  CoraLite®594
规格价格库存

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经过测试的应用

Positive IF/ICC detected inHeLa cells, HepG2 cells
Positive FC (Intra) detected inHeLa cells
Planning an IHC experiment? We recommend our IHCeasy GOLM1 Ready-To-Use IHC Kit. GOLM1 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 66331-1-Ig

推荐稀释比

应用推荐稀释比
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

产品信息

CL594-66331 targets GP73/GOLPH2 in IF/ICC, FC (Intra) applications and shows reactivity with human samples.

经测试应用 IF/ICC, FC (Intra) Application Description
经测试反应性 human
免疫原

CatNo: Ag7207

Product name: Recombinant human GOLM1 protein

Source: e coli.-derived, PGEX-4T

Tag: GST

Domain: 36-400 aa of BC001740

Sequence: SRSVDLQTRIMELEGRVRRAAAERGAVELKKNEFQGELEKQREQLDKIQSSHNFQLESVNKLYQDEKAVLVNNITTGERLIRVLQDQLKTLQRNYGRLQQDVLQFQKNQTNLERKFSYDLSQCINQMKEVKEQCEERIEEVTKKGNEAVASRDLSENNDQRQQLQALSEPQPRLQAAGLPHTEVPQGKGNVLGNSKSQTPAPSSEVVLDSKRQVEKEETNEIQVVNEEPQRDRLPQEPGREQVVEDRPVGGRGFGGAGELGQTPQVQAALSVSQENPEMEGPERDQLVIPDGQEEEQEAAGEGRNQQKLRGEDDYNMDENEAESETDKQAALAGNDRNIDVFNVEDQKRDTINLLDQREKRNHTL

种属同源性预测
宿主/亚型 Mouse / IgG1
抗体类别 Monoclonal
产品类型 Antibody
全称 golgi membrane protein 1
别名 GOLM1, GOLPH2, GP73, 3D1F1, C9orf155
计算分子量 45 kDa
GenBank蛋白编号BC001740
基因名称 GP73
Gene ID (NCBI) 51280
RRIDAB_2883560
偶联类型 CoraLite®594 Fluorescent Dye
最大激发/发射波长588 nm / 604 nm
形式Liquid
纯化方式Protein G purification
UNIPROT IDQ8NBJ4
储存缓冲液 PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
储存条件Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

背景介绍

Golgi phosphoprotein 2 (GOLPH2, also known as GP73 or GOLM1) is a resident Golgi type-II membrane protein. It is predominantly expressed in the epithelial cells of many human tissues. GOLPH2 traffics through endosomes and can be secreted into the circulation. Its expression is upregulated in a number of tumors and GOLPH2 could be a promising serum marker for hepatocellular carcinoma. We got 73 kDa in western blotting due to phosphorylation.

实验方案

Product Specific Protocols
FC protocol for CL594 GP73/GOLPH2 antibody CL594-66331Download protocol
IF protocol for CL594 GP73/GOLPH2 antibody CL594-66331Download protocol
Standard Protocols
Click here to view our Standard Protocols
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