验证数据展示
经过测试的应用
| Positive IF/ICC detected in | U2OS cells |
Planning an IHC experiment? We recommend our IHCeasy GORASP2 Ready-To-Use IHC Kit. GORASP2 primary antibody included.
For other applications, we recommend the unconjugated version of this antibody, 85077-5-PBS
推荐稀释比
| 应用 | 推荐稀释比 |
|---|---|
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
产品信息
CL594-85077-5 targets GORASP2 in applications and shows reactivity with human samples.
| 经测试应用 | IF/ICC Application Description |
| 经测试反应性 | human |
| 免疫原 | GORASP2 fusion protein Ag0950 种属同源性预测 |
| 宿主/亚型 | Rabbit / IgG |
| 抗体类别 | Recombinant |
| 产品类型 | Antibody |
| 全称 | golgi reassembly stacking protein 2, 55kDa |
| 别名 | 242700C11, Golgi phosphoprotein 6, Golgi reassembly-stacking protein 2, Golgi reassembly-stacking protein of 55 kDa, GOLPH6 |
| 计算分子量 | 55 kDa |
| 观测分子量 | 59 kDa |
| GenBank蛋白编号 | BC007770 |
| 基因名称 | GORASP2 |
| Gene ID (NCBI) | 26003 |
| 偶联类型 | CoraLite®594 Fluorescent Dye |
| 最大激发/发射波长 | 588 nm / 604 nm |
| 形式 | Liquid |
| 纯化方式 | Protein A purification |
| UNIPROT ID | Q9H8Y8 |
| 储存缓冲液 | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| 储存条件 | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
背景介绍
GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.
实验方案
| Product Specific Protocols | |
|---|---|
| IF protocol for CL594 GORASP2 antibody CL594-85077-5 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |