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Host
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Species Reactivity
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Species Reactivity
0
Conjugate
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Conjugate
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验证数据展示

  • WB analysis using 85077-5-RR (same clone as 85077-5-PBS)

    WB analysis using 85077-5-RR (same clone as 85077-5-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 85077-5-RR (GORASP2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

  • IHC staining of human colon cancer using 85077-5-RR (same clone as 85077-5-PBS)

    IHC staining of human colon cancer using 85077-5-RR (same clone as 85077-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GORASP2 antibody included
  • IHC staining of human colon cancer using 85077-5-RR (same clone as 85077-5-PBS)

    IHC staining of human colon cancer using 85077-5-RR (same clone as 85077-5-PBS)

    Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 85077-5-RR (GORASP2 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

    Ready-to-use IHC kits available with GORASP2 antibody included
  • IF Staining of HepG2 using 85077-5-RR (same clone as 85077-5-PBS)

    IF Staining of HepG2 using 85077-5-RR (same clone as 85077-5-PBS)

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using GORASP2 antibody (85077-5-RR, Clone: 242700C11 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 85077-5-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 85077-5-RR

    Affinity and Kinetic Characterization of 85077-5-RR

    Biolayer interferometry (BLl) kinetic assays of 85077-5-RR against Human GORASP2 were performed. The affinity constant is 5.33 nM.

GORASP2 Recombinant antibody, PBS Only

GORASP2 Uni-rAbTM Recombinant Antibody for WB, IHC, IF/ICC, Indirect ELISA
Cat No. 85077-5-PBS

产品说明书

CloneNo. 242700C11

宿主/亚型

Rabbit / IgG

种属反应性

human

应用

WB, IHC, IF/ICC, Indirect ELISA

别名

GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2, Golgi phosphoprotein 6

缓冲液配方:  PBS Only
PBS and Azide
PBS Only
偶联物:  Unconjugated
规格: 

-/ -

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Planning an IHC experiment? We recommend our IHCeasy GORASP2 Ready-To-Use IHC Kit. GORASP2 primary antibody included.

产品信息

85077-5-PBS targets GORASP2 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human samples.

经测试应用 WB, IHC, IF/ICC, Indirect ELISA Application Description
经测试反应性 human
免疫原 GORASP2 fusion protein Ag0950 种属同源性预测
宿主/亚型 Rabbit / IgG
抗体类别 Recombinant
产品类型 Antibody
全称 golgi reassembly stacking protein 2, 55kDa
别名 GRASP55, GOLPH6, Golgi reassembly-stacking protein of 55 kDa, Golgi reassembly-stacking protein 2, Golgi phosphoprotein 6
计算分子量 55 kDa
观测分子量 59 kDa
GenBank蛋白编号BC007770
基因名称 GORASP2
Gene ID (NCBI) 26003
偶联类型 Unconjugated
形式 Liquid
纯化方式Protein A purification
UNIPROT IDQ9H8Y8
储存缓冲液 PBS only , pH 7.3
储存条件Store at -80°C.
The product is shipped with ice packs. Upon receipt, store it immediately at -80°C

背景介绍

GORASP2 (Golgi reassembly-stacking protein 2, or GRS2) has multiple phosphorylation sites and can be phosphorylated by MKK/ERK pathway during mitoisis. GORASP2 plays a role in the assembly and membrane stacking of the Golgi cisternae, and in the process by which Golgi stacks reform after mitotic breakdown.GORASP2 is supposed to regulate the intracellular transport and presentation of a defined set of transmembrane proteins, such as transmembrane TGFA.